Literature DB >> 1749819

Low cost device for electrotransformation and its application to the highly efficient transformation of Escherichia coli and Enterococcus faecalis.

S Fujimoto1, H Hashimoto, Y Ike.   

Abstract

A simple, low cost device for electrotransformation has been designed and constructed. The cost of the circuit was only $150. Maximum field strength of 12,000 V/cm with an actual time constant up to 11 msec was obtained with a newly designed circuitry and a 0.1-cm electrode gap cuvette. Eschericia coli strains DH1, DH5 alpha, and LE392 were transformed at an efficiency of 10(9)/micrograms DNA with plasmids pUC18 and pBR322. E. faecalis strain OG1X was transformed at an efficiency of 0.9 x 10(5)/micrograms DNA without treatment with glycine.

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Year:  1991        PMID： 1749819     DOI： 10.1016/0147-619x(91)90053-y

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  19 in total

1.  Genetic analysis of transfer-related regions of the vancomycin resistance Enterococcus conjugative plasmid pHTbeta: identification of oriT and a putative relaxase gene.

Authors:  Haruyoshi Tomita; Yasuyoshi Ike
Journal:  J Bacteriol       Date:  2005-11       Impact factor: 3.490

2.  Cloning and genetic organization of the bacteriocin 31 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI17.

Authors:  H Tomita; S Fujimoto; K Tanimoto; Y Ike
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

3.  High-level plasmid-mediated gentamicin resistance and pheromone response of plasmids present in clinical isolates of Enterococcus faecalis.

Authors:  M Shiojima; H Tomita; K Tanimoto; S Fujimoto; Y Ike
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

4.  Genetic organization and mode of action of a novel bacteriocin, bacteriocin 51: determinant of VanA-type vancomycin-resistant Enterococcus faecium.

Authors:  Hitoshi Yamashita; Haruyoshi Tomita; Takako Inoue; Yasuyoshi Ike
Journal:  Antimicrob Agents Chemother       Date:  2011-06-27       Impact factor: 5.191

5.  Bac 32, a novel bacteriocin widely disseminated among clinical isolates of Enterococcus faecium.

Authors:  Takako Inoue; Haruyoshi Tomita; Yasuyoshi Ike
Journal:  Antimicrob Agents Chemother       Date:  2006-04       Impact factor: 5.191

6.  Physical mapping of the conjugative bacteriocin plasmid pPD1 of Enterococcus faecalis and identification of the determinant related to the pheromone response.

Authors:  S Fujimoto; H Tomita; E Wakamatsu; K Tanimoto; Y Ike
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

7.  pAM401-based shuttle vectors that enable overexpression of promoterless genes and one-step purification of tag fusion proteins directly from Enterococcus faecalis.

Authors:  S Fujimoto; Y Ike
Journal:  Appl Environ Microbiol       Date:  2001-03       Impact factor: 4.792

8.  Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gmr) (65.1 kilobases) to Enterococcus strains during broth mating.

Authors:  Y Ike; K Tanimoto; H Tomita; K Takeuchi; S Fujimoto
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

9.  Cloning and genetic analyses of the bacteriocin 41 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pYI14: a novel bacteriocin complemented by two extracellular components (lysin and activator).

Authors:  Haruyoshi Tomita; Elizabeth Kamei; Yasuyoshi Ike
Journal:  J Bacteriol       Date:  2008-01-18       Impact factor: 3.490

10.  Evidence that the hemolysin/bacteriocin phenotype of Enterococcus faecalis subsp. zymogenes can be determined by plasmids in different incompatibility groups as well as by the chromosome.

Authors:  Y Ike; D B Clewell
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490
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