Literature DB >> 17497279

Effects of Andrographitis Paniculata extracts on the expression of CD40 in endothelial cells.

Jie Zhang1, Shusheng Li, Lei Wan.   

Abstract

In order to investigate the expression of CD40 in endothelial cells (ECs) in a variety of injured conditions and the interventional role of Andrographitis Paniculata isolate (API(0134)), the thoracic aorta ECs of guinea pigs were cultured in vitro until the third passage, incubated in the presence of media containing xanthine oxidase (XO) and xanthine (Xan) which produced oxygen free radical (OFR group); oxidized-LDL (ox-LDL group); XO, Xan and API(0134) (OFR+API(0134) group); or ox-LDL and API(0134) (ox-LDL+API(0134) group). The expression of CD40 in ECs was detected by immunofluorescence assay and reverse transcription-PCR (RT-PCR). The results showed as compared with the control group, the expression of CD40 in ECs in OFR group and ox-LDL group was increased (P<0.01), but attenuated significantly in OFR+ API(0134) group and ox-LDL+API(0134) group (P<0.05). It was suggested that API(0134) could protect atherosclerosis by inhibiting the expression of CD40 molecule in injured ECs.

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Year:  2007        PMID： 17497279     DOI： 10.1007/s11596-007-0207-x

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  5 in total

1.  Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: implications for CD40-CD40 ligand signaling in atherosclerosis.

Authors:  F Mach; U Schönbeck; G K Sukhova; T Bourcier; J Y Bonnefoy; J S Pober; P Libby
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-04       Impact factor: 11.205

2.  C-reactive protein-induced expression of CD40-CD40L and the effect of lovastatin and fenofibrate on it in human vascular endothelial cells.

Authors:  Rong Lin; Juntian Liu; Weijie Gan; Guangde Yang
Journal:  Biol Pharm Bull       Date:  2004-10       Impact factor: 2.233

3.  [Culture of endotheliocytes derived from guinea-pig common carotid artery and establishment of guinea-pig donor model].

Authors:  Y Lai; W Shen; Y Li; C He; S Li
Journal:  Hua Xi Yi Ke Da Xue Xue Bao       Date:  2000-09

4.  LOX-1, an oxidized LDL endothelial receptor, induces CD40/CD40L signaling in human coronary artery endothelial cells.

Authors:  Dayuan Li; Ling Liu; Hongjiang Chen; Tatsuya Sawamura; Jawahar L Mehta
Journal:  Arterioscler Thromb Vasc Biol       Date:  2003-03-13       Impact factor: 8.311

5.  Prevention of atherosclerotic arterial stenosis and restenosis after angioplasty with Andrographis paniculata nees and fish oil. Experimental studies of effects and mechanisms.

Authors:  D W Wang; H Y Zhao
Journal:  Chin Med J (Engl)       Date:  1994-06       Impact factor: 2.628
  5 in total
  1 in total

1.  Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells.

Authors:  Fu-xing Li; Shu-sheng Li
Journal:  Chin J Integr Med       Date:  2011-04-26       Impact factor: 1.978
  1 in total

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