BACKGROUND: Mannose-binding lectin (MBL), a component of the innate immune system, provides a first-line defense against invading microorganisms. Polymorphisms in the MBL gene have been associated with increased risk of infection. Chlamydia trachomatis genital tract infections are a major cause of Fallopian tube occlusion. Our objective was to test whether an MBL codon 54 polymorphism might contribute to development of C. trachomatis-associated tubal damage. METHODS: In a case-control study, 97 women with occluded and 104 women with patent Fallopian tubes were tested for a history of chlamydial infection by serology and for their MBL codon 54 genotype by PCR and restriction fragment length polymorphism analysis. Clinical data were blinded to those performing all laboratory analyses. RESULTS: Women with tubal occlusion who also had a positive chlamydial serology had the highest rate of variant MBL B allele carriage (P<0.001). Among women who were chlamydial antibody negative, allele B carriage was also more frequent in those with blocked, as opposed to patent, Fallopian tubes (P<0.01). CONCLUSIONS: Wild-type allele A homozygosity is protective against, while carriage of the variant allele B is a risk factor for, Fallopian tube occlusion in women who are seropositive or seronegative for C. trachomatis.
BACKGROUND:Mannose-binding lectin (MBL), a component of the innate immune system, provides a first-line defense against invading microorganisms. Polymorphisms in the MBL gene have been associated with increased risk of infection. Chlamydia trachomatis genital tract infections are a major cause of Fallopian tube occlusion. Our objective was to test whether an MBL codon 54 polymorphism might contribute to development of C. trachomatis-associated tubal damage. METHODS: In a case-control study, 97 women with occluded and 104 women with patent Fallopian tubes were tested for a history of chlamydial infection by serology and for their MBL codon 54 genotype by PCR and restriction fragment length polymorphism analysis. Clinical data were blinded to those performing all laboratory analyses. RESULTS:Women with tubal occlusion who also had a positive chlamydial serology had the highest rate of variant MBL B allele carriage (P<0.001). Among women who were chlamydial antibody negative, allele B carriage was also more frequent in those with blocked, as opposed to patent, Fallopian tubes (P<0.01). CONCLUSIONS: Wild-type allele A homozygosity is protective against, while carriage of the variant allele B is a risk factor for, Fallopian tube occlusion in women who are seropositive or seronegative for C. trachomatis.
Authors: Rachel J Gorwitz; Harold C Wiesenfeld; Pai-Lien Chen; Karen R Hammond; Karen A Sereday; Catherine L Haggerty; Robert E Johnson; John R Papp; Dmitry M Kissin; Tara C Henning; Edward W Hook; Michael P Steinkampf; Lauri E Markowitz; William M Geisler Journal: Am J Obstet Gynecol Date: 2017-05-19 Impact factor: 8.661
Authors: M A F Queiroz; S T M Gomes; N C C Almeida; M I M Souza; S R C F Costa; R B Hermes; S S Lima; M M Zaninotto; M A A Fossa; M A Maneschy; R N Martins-Feitosa; V N Azevedo; L F A Machado; M O G Ishak; R Ishak; A C R Vallinoto Journal: Braz J Med Biol Res Date: 2016-12-12 Impact factor: 2.590
Authors: B M Hoenderboom; A A M van Oeffelen; B H B van Benthem; J E A M van Bergen; N H T M Dukers-Muijrers; H M Götz; C J P A Hoebe; A A Hogewoning; F R M van der Klis; D van Baarle; J A Land; M A B van der Sande; M G van Veen; F de Vries; S A Morré; I V F van den Broek Journal: BMC Infect Dis Date: 2017-04-11 Impact factor: 3.090
Authors: Stephan P Verweij; Remco P H Peters; Arnold Catsburg; Henry J C de Vries; Sander Ouburg; Servaas A Morré Journal: Int J Mol Sci Date: 2022-08-18 Impact factor: 6.208