Literature DB >> 17487210

Culture of human mast cells from peripheral blood progenitors.

Hirohisa Saito1, Atsushi Kato, Kenji Matsumoto, Yoshimichi Okayama.   

Abstract

This protocol details a method for obtaining a considerable number of human mast cells from peripheral blood cells from normal donors without using stem cell mobilization treatment. By using the magnetic cell sorting system, 10(4)-10(5) cells are retrieved in the CD34+ fraction when 100 ml of blood is drawn from a healthy donor. When these cells are cultured using methylcellulose medium supplemented with stem cell factor and interleukin 6, 10(3)-10(4) mast cell colonies are formed in 6 weeks. The total mast cell number at 6 weeks of culture will be 10(6)-10(7).

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Year:  2006        PMID： 17487210     DOI： 10.1038/nprot.2006.344

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  23 in total

1.  Lipoteichoic acid improves the capability of mast cells in the host defense system against bacteria.

Authors:  Naoki Imajo; Daisuke Kurihara; Nobuyuki Fukuishi; Asumi Inukai; Shinobu Matsushita; Shingo Noda; Mako Toyoda; Mino Yoshioka; Hayato Teruya; Yumiko Nishii; Nobuaki Matsui; Masaaki Akagi
Journal:  Inflamm Res       Date:  2009-05-26       Impact factor: 4.575

2.  RBL-2H3 cells are an imprecise model for mast cell mediator release.

Authors:  Egle Passante; Carsten Ehrhardt; Helen Sheridan; Neil Frankish
Journal:  Inflamm Res       Date:  2009-03-20       Impact factor: 4.575

3.  An immunoglobulin-like receptor, Allergin-1, inhibits immunoglobulin E-mediated immediate hypersensitivity reactions.

Authors:  Kaori Hitomi; Satoko Tahara-Hanaoka; Satoru Someya; Akira Fujiki; Hideaki Tada; Tetsuya Sugiyama; Shiro Shibayama; Kazuko Shibuya; Akira Shibuya
Journal:  Nat Immunol       Date:  2010-06-06       Impact factor: 25.606

4.  An optimized protocol for the generation and functional analysis of human mast cells from CD34+ enriched cell populations.

Authors:  Yuzhi Yin; Yun Bai; Ana Olivera; Avanti Desai; Dean D Metcalfe
Journal:  J Immunol Methods       Date:  2017-06-16       Impact factor: 2.303

5.  KTN0158, a Humanized Anti-KIT Monoclonal Antibody, Demonstrates Biologic Activity against both Normal and Malignant Canine Mast Cells.

Authors:  Cheryl A London; Heather L Gardner; Sarah Rippy; Gerald Post; Krista La Perle; Linda Crew; Lori Lopresti-Morrow; Andrew J Garton; Gerald McMahon; Theresa M LaVallee; Richard Gedrich
Journal:  Clin Cancer Res       Date:  2016-11-04       Impact factor: 12.531

6.  Novel six-week protocol for generating functional human connective tissue-type (MCTC) mast cells from buffy coats.

Authors:  Issan Yee San Tam; Chun Wai Ng; See-Ying Tam; Hang Yung Alaster Lau
Journal:  Inflamm Res       Date:  2016-09-15       Impact factor: 4.575

7.  Derivation of induced pluripotent stem cells from human peripheral blood T lymphocytes.

Authors:  Matthew E Brown; Elizabeth Rondon; Deepika Rajesh; Amanda Mack; Rachel Lewis; Xuezhu Feng; Laura Jo Zitur; Randall D Learish; Emile F Nuwaysir
Journal:  PLoS One       Date:  2010-06-29       Impact factor: 3.240

8.  Dexamethasone and FK506 inhibit expression of distinct subsets of chemokines in human mast cells.

Authors:  Atsushi Kato; Regina T Chustz; Takahisa Ogasawara; Marianna Kulka; Hirohisa Saito; Robert P Schleimer; Kenji Matsumoto
Journal:  J Immunol       Date:  2009-06-01       Impact factor: 5.422

9.  Mechanisms and biomarkers of inflammatory endotypes in chronic rhinosinusitis without nasal polyps.

Authors:  Aiko I Klingler; Whitney W Stevens; Bruce K Tan; Anju T Peters; Julie A Poposki; Leslie C Grammer; Kevin C Welch; Stephanie S Smith; David B Conley; Robert C Kern; Robert P Schleimer; Atsushi Kato
Journal:  J Allergy Clin Immunol       Date:  2020-12-14       Impact factor: 10.793

10.  Mast cell chemotaxis - chemoattractants and signaling pathways.

Authors:  Ivana Halova; Lubica Draberova; Petr Draber
Journal:  Front Immunol       Date:  2012-05-25       Impact factor: 7.561
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