AIM: Retinoic acid-inducible gene-I (RIG-I) is one of the genes induced by interferon (IFN)-gamma which plays an important role in atherosclerosis. The aim of this study is to examine if RIG-I is involved in atherosclerosis. METHODS: The expression of RIG-I in atherosclerotic lesions in human aorta was examined by immunohistochemical analysis. The expression of RIG-I in THP-1 monocytic cell line or human monocyte-derived macrophages was studied by western blot and RT-PCR analyses. RESULTS: Intense immunoreactivity for RIG-I was detected in intimal macrophages in atherosclerotic lesions. IFN-gamma slightly enhanced the RIG-I expression in THP-1 cells. Treatment of the cells with phorbol 12-myristate 13-acetate, which induces the differentiation of the cells into macrophage-like cells, significantly enhanced the IFN-gamma -induced RIG-I expression. IFN-gamma also stimulated the expression of RIG-I in monocyte-derived macrophages. CONCLUSION: These results suggest that RIG-I may be involved in differentiation and activation of macrophages, playing a role in atherosclerosis.
AIM: Retinoic acid-inducible gene-I (RIG-I) is one of the genes induced by interferon (IFN)-gamma which plays an important role in atherosclerosis. The aim of this study is to examine if RIG-I is involved in atherosclerosis. METHODS: The expression of RIG-I in atherosclerotic lesions in human aorta was examined by immunohistochemical analysis. The expression of RIG-I in THP-1 monocytic cell line or human monocyte-derived macrophages was studied by western blot and RT-PCR analyses. RESULTS: Intense immunoreactivity for RIG-I was detected in intimal macrophages in atherosclerotic lesions. IFN-gamma slightly enhanced the RIG-I expression in THP-1 cells. Treatment of the cells with phorbol 12-myristate 13-acetate, which induces the differentiation of the cells into macrophage-like cells, significantly enhanced the IFN-gamma -induced RIG-I expression. IFN-gamma also stimulated the expression of RIG-I in monocyte-derived macrophages. CONCLUSION: These results suggest that RIG-I may be involved in differentiation and activation of macrophages, playing a role in atherosclerosis.
Authors: Wolfgang Lieb; Justin P Zachariah; Vanessa Xanthakis; Radwan Safa; Ming-Huei Chen; Lisa M Sullivan; Martin G Larson; Holly M Smith; Qiong Yang; Gary F Mitchell; Joseph A Vita; Douglas B Sawyer; Ramachandran S Vasan Journal: Circ Cardiovasc Genet Date: 2010-03-26
Authors: T Imaizumi; T Arikawa; T Sato; R Uesato; T Matsumiya; H Yoshida; M Ueno; S Yamasaki; T Nakajima; M Hirashima; K Sakata; Y Ishibashi; S Toh; C Ohyama; K Satoh Journal: Clin Exp Immunol Date: 2008-05-26 Impact factor: 4.330
Authors: Tobias Asdonk; Martin Steinmetz; Alexander Krogmann; Christine Ströcker; Catharina Lahrmann; Inga Motz; Kathrin Paul-Krahe; Anna Flender; Theresa Schmitz; Winfried Barchet; Gunther Hartmann; Georg Nickenig; Sebastian Zimmer Journal: J Cell Mol Med Date: 2016-04-29 Impact factor: 5.310