Literature DB >> 17485067

Real-time PCR assay for the identification of cutaneous Leishmania parasite species in Constantine region of Algeria.

Frédérique de Monbrison1, Ilhem Mihoubi, Stéphane Picot.   

Abstract

We evaluated the use of real-time PCR for the identification of cutaneous Leishmania species. The assay, based on the melting curve analysis of fluorescent products, allows the discrimination of four culture adapted strains (L. infantum MHOM/TN/80/IPT1, L. major MHOM/SU/73/5-ASKH, L. donovani MHOM/IN/80/DD8 and L. tropica MHOM/SU/74/K27). One hundred and twenty-nine skin lesions, spotted on filter paper, were collected from patients consulting for suspicion of cutaneous leishmaniasis (CL) at the parasitology laboratory of Constantine Hospital (Algeria). Ninety-seven (75.2%) of the samples analyzed were positive. Sixty-one (5%) were related to L. major strain. These results indicate that PCR assay provides pleasant results with filter paper and represents a tool for the identification of old world CL.

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Year:  2007        PMID： 17485067     DOI： 10.1016/j.actatropica.2007.04.001

Source DB:  PubMed          Journal:  Acta Trop        ISSN： 0001-706X            Impact factor:   3.112


  7 in total

1.  Diagnosis and identification of Leishmania spp. from Giemsa-stained slides, by real-time PCR and melting curve analysis in south-west of Iran.

Authors:  S Khademvatan; N Neisi; S Maraghi; J Saki
Journal:  Ann Trop Med Parasitol       Date:  2011-12

2.  A FRET-based real-time PCR assay to identify the main causal agents of New World tegumentary leishmaniasis.

Authors:  Pablo Tsukayama; Jorge H Núñez; Maxy De Los Santos; Valeria Soberón; Carmen M Lucas; Greg Matlashewski; Alejandro Llanos-Cuentas; Marianela Ore; G Christian Baldeviano; Kimberly A Edgel; Andres G Lescano; Paul C F Graf; David J Bacon
Journal:  PLoS Negl Trop Dis       Date:  2013-01-03

3.  Comparison of three methods for diagnosis of cutaneous leishmaniasis.

Authors:  B Pourmohammadi; Mh Motazedian; Gr Hatam; M Kalantari; P Habibi; B Sarkari
Journal:  Iran J Parasitol       Date:  2010-12       Impact factor: 1.012

4.  Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.

Authors:  Carolina Hernández; Catalina Alvarez; Camila González; Martha Stella Ayala; Cielo Maritza León; Juan David Ramírez
Journal:  Parasit Vectors       Date:  2014-11-14       Impact factor: 3.876

5.  Real-time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation.

Authors:  Milli Nath-Chowdhury; Mugundhine Sangaralingam; Patrick Bastien; Christophe Ravel; Francine Pratlong; Juan Mendez; Michael Libman; Momar Ndao
Journal:  Parasit Vectors       Date:  2016-05-03       Impact factor: 3.876

6.  The use of kDNA minicircle subclass relative abundance to differentiate between Leishmania (L.) infantum and Leishmania (L.) amazonensis.

Authors:  Marcello Ceccarelli; Luca Galluzzi; Aurora Diotallevi; Francesca Andreoni; Hailie Fowler; Christine Petersen; Fabrizio Vitale; Mauro Magnani
Journal:  Parasit Vectors       Date:  2017-05-16       Impact factor: 3.876

Review 7.  Real-time PCR applications for diagnosis of leishmaniasis.

Authors:  Luca Galluzzi; Marcello Ceccarelli; Aurora Diotallevi; Michele Menotta; Mauro Magnani
Journal:  Parasit Vectors       Date:  2018-05-02       Impact factor: 3.876
  7 in total

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