Literature DB >> 17483227

Mutagenesis and molecular modeling reveal three key extracellular loops of the membrane receptor HasR that are involved in hemophore HasA binding.

Clément Barjon1, Karine Wecker, Nadia Izadi-Pruneyre, Philippe Delepelaire.   

Abstract

On the basis of the three-dimensional model of the heme/hemophore TonB-dependent outer membrane receptor HasR, mutants with six-residue deletions in the 11 putative extracellular loops were generated. Although all mutants continued to be active TonB-dependent heme transporters, mutations in three loops abolished hemophore HasA binding both in vivo and in vitro.

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Year:  2007        PMID： 17483227      PMCID： PMC1951882          DOI： 10.1128/JB.00251-07

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  The crystal structure of HasA, a hemophore secreted by Serratia marcescens.

Authors:  P Arnoux; R Haser; N Izadi; A Lecroisey; M Delepierre; C Wandersman; M Czjzek
Journal:  Nat Struct Biol       Date:  1999-06

Review 2.  Comparative protein structure modeling of genes and genomes.

Authors:  M A Martí-Renom; A C Stuart; A Fiser; R Sánchez; F Melo; A Sali
Journal:  Annu Rev Biophys Biomol Struct       Date:  2000

3.  A graph-theory algorithm for rapid protein side-chain prediction.

Authors:  Adrian A Canutescu; Andrew A Shelenkov; Roland L Dunbrack
Journal:  Protein Sci       Date:  2003-09       Impact factor: 6.725

4.  Ligand delivery by haem carrier proteins: the binding of Serratia marcescens haemophore to its outer membrane receptor is mediated by two distinct peptide regions.

Authors:  Sylvie Létoffé; Laurent Debarbieux; Nadia Izadi; Philippe Delepelaire; Cécile Wandersman
Journal:  Mol Microbiol       Date:  2003-10       Impact factor: 3.501

5.  Activities of the Serratia marcescens heme receptor HasR and isolated plug and beta-barrel domains: the beta-barrel forms a heme-specific channel.

Authors:  Sylvie Létoffé; Karine Wecker; Muriel Delepierre; Philippe Delepelaire; Cécile Wandersman
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

6.  Purification, crystallization and preliminary X-ray analysis of the outer membrane complex HasA-HasR from Serratia marcescens.

Authors:  Frédéric Huché; Philippe Delepelaire; Cécile Wandersman; Wolfram Welte
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2005-12-16

7.  Multiple extracellular loops contribute to substrate binding and transport by the Escherichia coli cobalamin transporter BtuB.

Authors:  Cynthia A Fuller-Schaefer; Robert J Kadner
Journal:  J Bacteriol       Date:  2005-03       Impact factor: 3.490

8.  The heme transfer from the soluble HasA hemophore to its membrane-bound receptor HasR is driven by protein-protein interaction from a high to a lower affinity binding site.

Authors:  Nadia Izadi-Pruneyre; Frédéric Huché; Gudrun S Lukat-Rodgers; Anne Lecroisey; Robert Gilli; Kenton R Rodgers; Cécile Wandersman; Philippe Delepelaire
Journal:  J Biol Chem       Date:  2006-06-14       Impact factor: 5.157

9.  Effect of loop deletions on the binding and transport of ferric enterobactin by FepA.

Authors:  S M Newton; J D Igo; D C Scott; P E Klebba
Journal:  Mol Microbiol       Date:  1999-06       Impact factor: 3.501

10.  Comparative structural analysis of TonB-dependent outer membrane transporters: implications for the transport cycle.

Authors:  David P Chimento; Robert J Kadner; Michael C Wiener
Journal:  Proteins       Date:  2005-05-01
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  3 in total

1.  Structural characterization of the hemophore HasAp from Pseudomonas aeruginosa: NMR spectroscopy reveals protein-protein interactions between Holo-HasAp and hemoglobin.

Authors:  Aileen Y Alontaga; Juan Carlos Rodriguez; Ernst Schönbrunn; Andreas Becker; Todd Funke; Erik T Yukl; Takahiro Hayashi; Jordan Stobaugh; Pierre Moënne-Loccoz; Mario Rivera
Journal:  Biochemistry       Date:  2009-01-13       Impact factor: 3.162

2.  Heme uptake across the outer membrane as revealed by crystal structures of the receptor-hemophore complex.

Authors:  Stefanie Krieg; Frédéric Huché; Kay Diederichs; Nadia Izadi-Pruneyre; Anne Lecroisey; Cécile Wandersman; Philippe Delepelaire; Wolfram Welte
Journal:  Proc Natl Acad Sci U S A       Date:  2009-01-14       Impact factor: 11.205

3.  Extracellular heme uptake and the challenges of bacterial cell membranes.

Authors:  Aaron D Smith; Angela Wilks
Journal:  Curr Top Membr       Date:  2012       Impact factor: 3.049
  3 in total

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