Rahi Victory1, Ghassan M Saed, Michael P Diamond. 1. Department of Obstetrics and Gynecology, C.S. Mott Center, Wayne State University, Detroit, Michigan 48201, USA. rahivictory@yahoo.com
Abstract
OBJECTIVE: To determine whether docosahexaenoic acid (DHA) reduces adhesion marker mRNA levels in normal peritoneal and adhesion fibroblasts. DESIGN: Prospective experimental study. SETTING: University Medical Center. PATIENT(S): Three patients undergoing laparotomy with excision of adhesions and normal peritoneum. INTERVENTION(S): DHA treatment (100 muM) of cell cultures for 24 hours. MAIN OUTCOME MEASURE(S): Real-time reverse transcriptase polymerase chain reaction (RT-PCR) quantification of relative changes (mRNA copies/mug mRNA) in mRNA levels of type I collagen, vascular endothelial growth factor (VEGF), and transforming growth factor-beta1 (TGF-beta1). RESULT(S): The DHA treatment significantly reduced type I collagen and VEGF, but not TGF-beta1 mRNA levels in normal peritoneal fibroblasts compared to normal controls. The DHA treatment of adhesion fibroblasts reduced type I collagen mRNAs to those of normal peritoneal fibroblasts, decreasing mRNAs by 35% compared to untreated adhesion fibroblasts. The VEGF mRNA levels were 50% lower in DHA-treated adhesion fibroblasts versus untreated adhesion fibroblasts. Docasahexaenoic acid reduced TGF- beta1 mRNA to normal levels in treated adhesion fibroblasts compared to untreated normal peritoneal fibroblasts. CONCLUSION(S): Docasahexaenoic acid substantially reduces levels of adhesion-related markers in normal peritoneal and adhesion fibroblasts. This study provides the molecular basis for an easily administered and potentially, highly efficacious, antiadhesion adjuvant.
OBJECTIVE: To determine whether docosahexaenoic acid (DHA) reduces adhesion marker mRNA levels in normal peritoneal and adhesion fibroblasts. DESIGN: Prospective experimental study. SETTING: University Medical Center. PATIENT(S): Three patients undergoing laparotomy with excision of adhesions and normal peritoneum. INTERVENTION(S): DHA treatment (100 muM) of cell cultures for 24 hours. MAIN OUTCOME MEASURE(S): Real-time reverse transcriptase polymerase chain reaction (RT-PCR) quantification of relative changes (mRNA copies/mug mRNA) in mRNA levels of type I collagen, vascular endothelial growth factor (VEGF), and transforming growth factor-beta1 (TGF-beta1). RESULT(S): The DHA treatment significantly reduced type I collagen and VEGF, but not TGF-beta1 mRNA levels in normal peritoneal fibroblasts compared to normal controls. The DHA treatment of adhesion fibroblasts reduced type I collagen mRNAs to those of normal peritoneal fibroblasts, decreasing mRNAs by 35% compared to untreated adhesion fibroblasts. The VEGF mRNA levels were 50% lower in DHA-treated adhesion fibroblasts versus untreated adhesion fibroblasts. Docasahexaenoic acid reduced TGF- beta1 mRNA to normal levels in treated adhesion fibroblasts compared to untreated normal peritoneal fibroblasts. CONCLUSION(S): Docasahexaenoic acid substantially reduces levels of adhesion-related markers in normal peritoneal and adhesion fibroblasts. This study provides the molecular basis for an easily administered and potentially, highly efficacious, antiadhesion adjuvant.
Authors: Awoniyi O Awonuga; Jimmy Belotte; Suleiman Abuanzeh; Nicole M Fletcher; Michael P Diamond; Ghassan M Saed Journal: Reprod Sci Date: 2014-02-11 Impact factor: 3.060
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