BACKGROUND: Fulminant hepatitis and biliary atresia are serious problems and their causes have not been explained well. We investigated whether or not erythrovirus B19 is a candidate etiologic agent in such liver disease patients who had undergone liver transplantation. METHODS: Liver tissues from 47 patients consisted of 28 fulminant hepatitis and 19 biliary atresia were examined to detect B19 genes by PCR and further analyzed their genomic characterization. RESULTS: B19 DNA was detected by nested PCR in 10 of 28 cases (35.7%) livers in the fulminant hepatitis group and 7 of 19 (36.8%) livers in the biliary atresia group, respectively (statistically not significant). Importantly, among the 8 hepatic B19 DNA-positive patients who had paired samples of liver and serum, the serum B19 genome was detectable in only one case. B19 mRNA was identified in all of 10 fulminant hepatitis cases with hepatic B19 DNA, but only 1 out of 7 (14.3%) cases in biliary atresia tested. Furthermore, we obtained ten isolates having the B19 genome with nearly full-length sequences. Interestingly, phylogenetic analysis based on the NS1 gene revealed three different clusters: two for isolates from fulminant hepatitis and the other for isolates from biliary atresia. CONCLUSIONS: Our results presented here suggested that B19 may be an etiologic agent of fulminant hepatitis.
BACKGROUND:Fulminant hepatitis and biliary atresia are serious problems and their causes have not been explained well. We investigated whether or not erythrovirus B19 is a candidate etiologic agent in such liver diseasepatients who had undergone liver transplantation. METHODS: Liver tissues from 47 patients consisted of 28 fulminant hepatitis and 19 biliary atresia were examined to detect B19 genes by PCR and further analyzed their genomic characterization. RESULTS:B19 DNA was detected by nested PCR in 10 of 28 cases (35.7%) livers in the fulminant hepatitis group and 7 of 19 (36.8%) livers in the biliary atresia group, respectively (statistically not significant). Importantly, among the 8 hepatic B19 DNA-positive patients who had paired samples of liver and serum, the serum B19 genome was detectable in only one case. B19 mRNA was identified in all of 10 fulminant hepatitis cases with hepatic B19 DNA, but only 1 out of 7 (14.3%) cases in biliary atresia tested. Furthermore, we obtained ten isolates having the B19 genome with nearly full-length sequences. Interestingly, phylogenetic analysis based on the NS1 gene revealed three different clusters: two for isolates from fulminant hepatitis and the other for isolates from biliary atresia. CONCLUSIONS: Our results presented here suggested that B19 may be an etiologic agent of fulminant hepatitis.
Authors: M J Alter; M Gallagher; T T Morris; L A Moyer; E L Meeks; K Krawczynski; J P Kim; H S Margolis Journal: N Engl J Med Date: 1997-03-13 Impact factor: 91.245
Authors: W F Balistreri; R Grand; J H Hoofnagle; F J Suchy; F C Ryckman; D H Perlmutter; R J Sokol Journal: Hepatology Date: 1996-06 Impact factor: 17.425
Authors: Luciane Almeida Amado Leon; Renato Sergio Marchevsky; Ana Maria Coimbra Gaspar; Rita de Cassia Nasser Cubel Garcia; Adilson José de Almeida; Marcelo Pelajo-Machado; Tatiana Xavier de Castro; Jussara Pereira do Nascimento; Kevin E Brown; Marcelo Alves Pinto Journal: Mem Inst Oswaldo Cruz Date: 2016-04 Impact factor: 2.743