| Literature DB >> 17478097 |
Mo Zhao Lan1, Xiao Peng, Mao Yun Xiang, Zou Yu Xia, Wang Bo, Li Jie, Xu Yong Li, Zhang Pei Jun.
Abstract
The esrB gene of Edwardsiella tarda, which encodes a regulator protein of the type III secretion system, was mutated by the unmarked deletion method and reintroduced by allelic exchange into the chromosome of E. tarda LSE40 by means of the suicide vector pRE112. The LSE40 esrB mutant was highly attenuated when inoculated intraperitoneally into turbot Scophthamus maximus L., showing a 50% lethal dose of 10(8.1)cfu/fish. The esrB mutants were not recoverable from the internal organs at 14 days post-inoculation. Vaccination with a single dose of 10(5)-10(7) cfu/fish of the esrB mutant elicited significant protection against the wild-type strain of E. tarda LSE40 (relative percentage survival>50%). The protection correlated well with the antibody titres in the serum of vaccinated fish.Entities:
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Year: 2006 PMID： 17478097 DOI： 10.1016/j.fsi.2006.11.002
Source DB: PubMed Journal: Fish Shellfish Immunol ISSN： 1050-4648 Impact factor: 4.581