| Literature DB >> 17472895 |
Andrew J Hampshire1, David A Rusling, Victoria J Broughton-Head, Keith R Fox.
Abstract
Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The method is based on the ability of the ligand to protect DNA from cleavage at its binding site. This review describes the use of DNase I and hydroxyl radicals, the most commonly used footprinting probes, in footprinting experiments. The success of a footprinting experiment depends on using an appropriate DNA substrate and we describe how these can best be chosen or designed. Although footprinting was originally developed for assessing a ligand's sequence selectivity, it can also be employed to estimate the binding strength (quantitative footprinting) and to assess the association and dissociation rate constants for slow binding reactions.Entities:
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Year: 2007 PMID： 17472895 DOI： 10.1016/j.ymeth.2007.01.002
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608