Literature DB >> 174717

Synchronous digestion of SV40 DNA by exonuclease III.

R Wu, G Ruben, B Siegel, E Jay, P Spielman, C P Tu.   

Abstract

We have established an optimal condition for the synchronous digestion of SV40 DNA with Escherichia coli exonuclease III. Electron microscopy and polyacrylamide gel electrophoresis were used to obtain accurate measurements on the lengths of DNA before and after exonuclease III digestion. Based on this finding, a new method for determining the sequence of long duplex DNA can be realized. It involves (a) the synchronous digestion of the DNA from the 3' ends with exonuclease III, followed by (b) repair synthesis with labeled nucleotides and DNA polymerase, and (c) sequence analysis of the repaired DNA.

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Year:  1976        PMID： 174717     DOI： 10.1021/bi00649a003

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  13 in total

1.  Regulatory elements and DNA-binding proteins mediating transcription from the chicken very-low-density apolipoprotein II gene.

Authors:  J M Beekman; J Wijnholds; I J Schippers; W Pot; M Gruber; G Ab
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

2.  Relaxed circular SV40 DNA as cleavage intermediate of two restriction endonucleases.

Authors:  G Ruben; P Spielman; C D Tu; E Jay; B Siegel; R Wu
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

3.  Ligation independent cloning irrespective of restriction site compatibility.

Authors:  C Li; R M Evans
Journal:  Nucleic Acids Res       Date:  1997-10-15       Impact factor: 16.971

4.  Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis.

Authors:  K L Nakamaye; F Eckstein
Journal:  Nucleic Acids Res       Date:  1986-12-22       Impact factor: 16.971

5.  An improved ligase-free method for directional subcloning of PCR amplified DNA.

Authors:  M Zhou; Z Hatahet
Journal:  Nucleic Acids Res       Date:  1995-03-25       Impact factor: 16.971

6.  Exoquence DNA sequencing.

Authors:  C Li; P W Tucker
Journal:  Nucleic Acids Res       Date:  1993-03-11       Impact factor: 16.971

7.  New rapid methods for DNA sequencing based in exonuclease III digestion followed by repair synthesis.

Authors:  L H Guo; R Wu
Journal:  Nucleic Acids Res       Date:  1982-03-25       Impact factor: 16.971

8.  Fusion of adenovirus E1A to the glucocorticoid receptor by high-resolution deletion cloning creates a hormonally inducible viral transactivator.

Authors:  D M Becker; S M Hollenberg; R P Ricciardi
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

9.  A general method for maximizing the expression of a cloned gene.

Authors:  T M Roberts; R Kacich; M Ptashne
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

10.  Deletion analysis of the CAP-cAMP binding site of the Escherichia coli lactose promoter.

Authors:  X M Yu; W S Reznikoff
Journal:  Nucleic Acids Res       Date:  1984-07-11       Impact factor: 16.971
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