OBJECTIVE: To submit different glove brands to double-quality control tests using mouse embryo assay (MEA) and the human sperm motility assay (HuSMA). Operator protection against infectious body fluid contamination is a safety issue in assisted reproductive technology (ART). When using gloves in the ART laboratory, toxic substances can be transmitted to culture media, even during brief contact. DESIGN: Quality control study of gloves in ART. SETTING: University hospital-based infertility center. ANIMAL(S): Seven- to 8-week-old female B6D2F1 hybrid mice. INTERVENTION(S): We tested two surgical, two cleanroom, and six examination glove brands. Only gloves brands that passed both HuSMA and MEA were submitted to further QC using zona-free and/or cryopreserved MEA. MAIN OUTCOME MEASURE(S): Sperm motility index, two-cell and blastocyst development, blastocyst total cell number. RESULT(S): Quality control by MEA and HuSMA identified two glove brands to be nontoxic. CONCLUSION(S): Our study shows that gloves used in ART can be toxic and should be tested as part of an ongoing quality control program.
OBJECTIVE: To submit different glove brands to double-quality control tests using mouse embryo assay (MEA) and the human sperm motility assay (HuSMA). Operator protection against infectious body fluid contamination is a safety issue in assisted reproductive technology (ART). When using gloves in the ART laboratory, toxic substances can be transmitted to culture media, even during brief contact. DESIGN: Quality control study of gloves in ART. SETTING: University hospital-based infertility center. ANIMAL(S): Seven- to 8-week-old female B6D2F1 hybrid mice. INTERVENTION(S): We tested two surgical, two cleanroom, and six examination glove brands. Only gloves brands that passed both HuSMA and MEA were submitted to further QC using zona-free and/or cryopreserved MEA. MAIN OUTCOME MEASURE(S): Sperm motility index, two-cell and blastocyst development, blastocyst total cell number. RESULT(S): Quality control by MEA and HuSMA identified two glove brands to be nontoxic. CONCLUSION(S): Our study shows that gloves used in ART can be toxic and should be tested as part of an ongoing quality control program.
Authors: Antoine Hannoun; Tony G Zreik; Ghina Ghaziri; Antoine Abu Musa; Johnny Awwad Journal: J Assist Reprod Genet Date: 2008-12-24 Impact factor: 3.412
Authors: Phoebe M Hughes; Dean E Morbeck; Susan B A Hudson; Jolene R Fredrickson; David L Walker; Charles C Coddington Journal: J Assist Reprod Genet Date: 2010-01-27 Impact factor: 3.412