| Literature DB >> 17466983 |
Nobuhiko Hiraga1, Michio Imamura, Masataka Tsuge, Chiemi Noguchi, Shoichi Takahashi, Eiji Iwao, Yoshifumi Fujimoto, Hiromi Abe, Toshiro Maekawa, Hidenori Ochi, Chise Tateno, Katsutoshi Yoshizato, Akihito Sakai, Yoshio Sakai, Masao Honda, Shuichi Kaneko, Takaji Wakita, Kazuaki Chayama.
Abstract
We developed a reverse genetics system of hepatitis C virus (HCV) genotypes 1a and 2a using infectious clones and human hepatocyte chimeric mice. We inoculated cell culture-produced genotype 2a (JFH-1) HCV intravenously. We also injected genotype 1a CV-H77C clone RNA intrahepatically. Mice inoculated with HCV by both procedures developed measurable and transmissible viremia. Interferon (IFN) alpha treatment resulted in greater reduction of genotype 2a HCV levels than genotype 1a, as seen in clinical practice. Genetically engineered HCV infection system should be useful for analysis of the mechanisms of resistance of HCV to IFN and other drugs.Entities:
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Year: 2007 PMID： 17466983 DOI： 10.1016/j.febslet.2007.04.021
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124