Zehra Hashim1, Shamshad Zarina. 1. Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan.
Abstract
OBJECTIVES: The purpose of this study was to evaluate antioxidant effect of paraoxonase 1 activity and malondialdehyde (MDA) levels as a marker of oxidative stress in patients suffering from cataract due to diabetes and aging. DESIGN AND METHODS: One hundred cataract patients (senile and diabetic) and age- and sex-matched controls were studied. Paraoxonase 1 and arylesterase activities in plasma samples were measured using paraoxon and phenylacetate as substrates, respectively. The magnitude of lipid peroxidation was established by measuring plasma MDA and oxidized low-density lipoprotein (ox LDL) levels. One-way ANOVA was employed for analysis of results. RESULTS: We observed significantly lower plasma paraoxonase and arylesterase activities in senile and diabetic cataractous patients as compared to respective controls (p<0.001). Plasma MDA and ox LDL levels were found to be higher in patients suffering from cataract (p<0.001). CONCLUSIONS: The results of present study suggest that the observed decrease in PON1 activity may be due to increase in oxidative stress. It can be concluded that lower paraoxonase activity could contribute to the higher risk of cataract formation.
OBJECTIVES: The purpose of this study was to evaluate antioxidant effect of paraoxonase 1 activity and malondialdehyde (MDA) levels as a marker of oxidative stress in patients suffering from cataract due to diabetes and aging. DESIGN AND METHODS: One hundred cataractpatients (senile and diabetic) and age- and sex-matched controls were studied. Paraoxonase 1 and arylesterase activities in plasma samples were measured using paraoxon and phenylacetate as substrates, respectively. The magnitude of lipid peroxidation was established by measuring plasma MDA and oxidized low-density lipoprotein (ox LDL) levels. One-way ANOVA was employed for analysis of results. RESULTS: We observed significantly lower plasma paraoxonase and arylesterase activities in senile and diabetic cataractouspatients as compared to respective controls (p<0.001). Plasma MDA and ox LDL levels were found to be higher in patients suffering from cataract (p<0.001). CONCLUSIONS: The results of present study suggest that the observed decrease in PON1 activity may be due to increase in oxidative stress. It can be concluded that lower paraoxonase activity could contribute to the higher risk of cataract formation.