Literature DB >> 17463

Enzymatic hydrolysis of agar: purification and characterization of beta-neoagarotetraose hydrolase from Pseudomonas atlantica.

D Groleau, W Yaphe.   

Abstract

Agarose is degraded by a beta-agarase from Pseudomonas atlantica to neoagarooligosaccharides of degree of polymerization (DP), 4, 6, 8, and 10. A beta-neoagarotetraose hydrolase cleaves the central beta-linkage in neoagarotetraose and the beta-linkage near the nonreducing end in neoagarohexaose and -octaose to yield neoagarobiose. The beta-neoagarotetraose hydrolase was localized on or outside the cytoplasmic membrane, in the cell wall region. The enzyme was activated by NaCl, KCl, CaCl2, MnCl2, and MgSO4, has a Km of 3.4 X 10(-3) M for neoagarotetraose, was free from beta-agarase and alpha-neoagarobiose hydrolase activity, and showed no transglycosidic activity.

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Year:  1977        PMID： 17463     DOI： 10.1139/m77-100

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN： 0008-4166            Impact factor:   2.419


  11 in total

1.  Purification and Properties of an Extracellular Agarase from Alteromonas sp. Strain C-1.

Authors:  O Leon; L Quintana; G Peruzzo; J C Slebe
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

2.  Cloning and Gene Replacement Mutagenesis of a Pseudomonas atlantica Agarase Gene.

Authors:  Robert Belas; Douglas Bartlett; Michael Silverman
Journal:  Appl Environ Microbiol       Date:  1988-01       Impact factor: 4.792

3.  Cloning, purification and biochemical characterization of beta agarase from the marine bacterium Pseudoalteromonas sp. AG4.

Authors:  Chulhong Oh; Chamilani Nikapitiya; Youngdeuk Lee; Ilson Whang; Se-Jae Kim; Do-Hyung Kang; Jehee Lee
Journal:  J Ind Microbiol Biotechnol       Date:  2010-03-07       Impact factor: 3.346

4.  Isolation of a unique marine bacterium capable of growth on a wide variety of polysaccharides from macroalgae.

Authors:  R S Quatrano; B A Caldwell
Journal:  Appl Environ Microbiol       Date:  1978-12       Impact factor: 4.792

5.  Identification and biochemical characterization of Sco3487 from Streptomyces coelicolor A3(2), an exo- and endo-type β-agarase-producing neoagarobiose.

Authors:  Uyangaa Temuujin; Won-Jae Chi; Yong-Keun Chang; Soon-Kwang Hong
Journal:  J Bacteriol       Date:  2011-10-21       Impact factor: 3.490

6.  Identification of a marine agarolytic pseudoalteromonas isolate and characterization of its extracellular agarase

Authors: 
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

Review 7.  Agarase: review of major sources, categories, purification method, enzyme characteristics and applications.

Authors:  Xiao Ting Fu; Sang Moo Kim
Journal:  Mar Drugs       Date:  2010-01-26       Impact factor: 5.118

8.  The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours.

Authors:  Murielle Jam; Didier Flament; Julie Allouch; Philippe Potin; Laurent Thion; Bernard Kloareg; Mirjam Czjzek; William Helbert; Gurvan Michel; Tristan Barbeyron
Journal:  Biochem J       Date:  2005-02-01       Impact factor: 3.857

9.  Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium, Vibrio sp. strain JT0107.

Authors:  Y Sugano; T Matsumoto; H Kodama; M Noma
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

10.  Molecular cloning, characterization and enzymatic properties of a novel βeta-agarase from a marine isolate Psudoalteromonas SP. AG52.

Authors:  Chulhong Oh; Chamilani Nikapitiya; Youngdeuk Lee; Ilson Whang; Do-Hyung Kang; Soo-Jin Heo; Young-Ung Choi; Jehee Lee
Journal:  Braz J Microbiol       Date:  2010-12-01       Impact factor: 2.476
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