| Literature DB >> 17458087 |
W Roszkowska-Jakimiec1, E Dabrowska, M Gacko, A Karwowska, M Chlabicz.
Abstract
The aim of the study was the demonstration and choice of conditions for the determination of cathepsin D activity in human mixed saliva. The 6% solution of hemoglobin, denatured with hydrochloric acid, was used as the substrate. The ratio of saliva volume to hemoglobin was 4:1 w/v. The reaction was interrupted by adding 10% trichloroacetic acid, after 6 hours of incubation at 37 degrees C. The increase in degradation products was determined with the use of Folina and Ciocalteau method with copper modification.Entities:
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Year: 2006 PMID： 17458087
Source DB: PubMed Journal: Adv Med Sci ISSN： 1896-1126 Impact factor: 3.287