Literature DB >> 17457673

Hyperoxidized peroxiredoxins and glyceraldehyde-3-phosphate dehydrogenase immunoreactivity and protein levels are changed in the gerbil hippocampal CA1 region after transient forebrain ischemia.

In Koo Hwang1, Ki-Yeon Yoo, Dae Won Kim, Jung Hoon Choi, In Se Lee, Moo Ho Won.   

Abstract

Oxidative stress is a major pathogenic event occurring in several brain disorders and is a major cause of brain damage due to ischemia/reperfusion. Thiol proteins are easily oxidized in cells exposed to reactive oxygen species (ROS). In the present study, we investigated transient ischemia-induced chronological changes in hyperoxidized peroxiredoxins (Prx-SO3) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH-SO3) immunoreactivity and protein levels in the gerbil hippocampus induced by 5 min of transient forebrain ischemia. Weak Prx-SO3 immunoreactivity is detected in the hippocampal CA1 region of the sham-operated group. Prx-SO3 immunoreactivity was significantly increased 12 h and 1 day after ischemia/reperfusion, and the immunoreactivity was decreased to the level of the sham-operated group 2 days after ischemia/reperfusion. Prx-SO3 immunoreactivity in the 4 days post-ischemia group was increased again, and the immunoreactivity was expressed in glial components for 5 days after ischemia/reperfusion. GAPDH-SO3 immunoreactivity was highest in the CA1 region 1 day after ischemia/reperfusion, the immunoreactivity was decreased 2 days after ischemia/reperfusion. Four days after ischemia/reperfusion, GAPDH-SO3 immunoreactivity increased again, and the immunoreactivity began to be expressed in glial components from 5 days after ischemia/reperfusion. Prx-SO3 and GAPDH-SO3 protein levels in the ischemic CA1 region were also very high 12 h and 1 day after ischemia/reperfusion and returned to the level of the sham-operated group 3 days after ischemia/reperfusion. Their protein levels were increased again 5 days after ischemia/reperfusion. In conclusion, Prx-SO3 and GAPDH-SO3 immunoreactivity and protein levels in the gerbil hippocampal CA1 region are significantly increased 12 h-24 h after ischemia/reperfusion and their immunoreactivity begins to be expressed in glial components from 4 or 5 days after ischemia/reperfusion.

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Year:  2007        PMID： 17457673     DOI： 10.1007/s11064-007-9345-6

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  36 in total

1.  Reversible oxidation of the active site cysteine of peroxiredoxins to cysteine sulfinic acid. Immunoblot detection with antibodies specific for the hyperoxidized cysteine-containing sequence.

Authors:  Hyun Ae Woo; Sang Won Kang; Hyung Ki Kim; Kap-Seok Yang; Ho Zoon Chae; Sue Goo Rhee
Journal:  J Biol Chem       Date:  2003-10-14       Impact factor: 5.157

2.  Proteomics analysis of cellular response to oxidative stress. Evidence for in vivo overoxidation of peroxiredoxins at their active site.

Authors:  Thierry Rabilloud; Manfred Heller; Francoise Gasnier; Sylvie Luche; Catherine Rey; Ruedi Aebersold; Mohamed Benahmed; Pierre Louisot; Joel Lunardi
Journal:  J Biol Chem       Date:  2002-03-19       Impact factor: 5.157

3.  Copper chaperone for Cu,Zn-SOD supplement potentiates the Cu,Zn-SOD function of neuroprotective effects against ischemic neuronal damage in the gerbil hippocampus.

Authors:  In Koo Hwang; Won Sik Eum; Ki-Yeon Yoo; Jun Hwi Cho; Dae Won Kim; Soo Hyun Choi; Tae-Cheon Kang; Oh-Shin Kwon; Jung Hoon Kang; Soo Young Choi; Moo Ho Won
Journal:  Free Radic Biol Med       Date:  2005-04-13       Impact factor: 7.376

4.  Delayed neuronal recovery and neuronal death in rat hippocampus following severe cerebral ischemia: possible relationship to abnormalities in neuronal processes.

Authors:  C K Petito; W A Pulsinelli
Journal:  J Cereb Blood Flow Metab       Date:  1984-06       Impact factor: 6.200

Review 5.  GAPDH as a sensor of NO stress.

Authors:  Makoto R Hara; Matthew B Cascio; Akira Sawa
Journal:  Biochim Biophys Acta       Date:  2006-03-09

Review 6.  Mitochondria, NO and neurodegeneration.

Authors:  M F Beal
Journal:  Biochem Soc Symp       Date:  1999

7.  Electron microscopic evidence against apoptosis as the mechanism of neuronal death in global ischemia.

Authors:  F Colbourne; G R Sutherland; R N Auer
Journal:  J Neurosci       Date:  1999-06-01       Impact factor: 6.167

Review 8.  Mitochondrial reactive oxygen species in cell death signaling.

Authors:  Christophe Fleury; Bernard Mignotte; Jean-Luc Vayssière
Journal:  Biochimie       Date:  2002 Feb-Mar       Impact factor: 4.079

9.  Hyperoxic sheep pulmonary microvascular endothelial cells generate free radicals via mitochondrial electron transport.

Authors:  S P Sanders; J L Zweier; P Kuppusamy; S J Harrison; D J Bassett; E W Gabrielson; J T Sylvester
Journal:  J Clin Invest       Date:  1993-01       Impact factor: 14.808

Review 10.  Structure, mechanism and regulation of peroxiredoxins.

Authors:  Zachary A Wood; Ewald Schröder; J Robin Harris; Leslie B Poole
Journal:  Trends Biochem Sci       Date:  2003-01       Impact factor: 13.807
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  4 in total

1.  Nuclear complex of glyceraldehyde-3-phosphate dehydrogenase and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxidant-induced cell death.

Authors:  Xuwei Hou; Patricia Snarski; Yusuke Higashi; Tadashi Yoshida; Alexander Jurkevich; Patrick Delafontaine; Sergiy Sukhanov
Journal:  FASEB J       Date:  2017-04-12       Impact factor: 5.191

2.  Transgenic overexpression of peroxiredoxin-2 attenuates ischemic neuronal injury via suppression of a redox-sensitive pro-death signaling pathway.

Authors:  Yu Gan; Xunming Ji; Xiaoming Hu; Yumin Luo; Lili Zhang; Peiying Li; Xiangrong Liu; Feng Yan; Peter Vosler; Yanqin Gao; R Anne Stetler; Jun Chen
Journal:  Antioxid Redox Signal       Date:  2012-05-14       Impact factor: 8.401

3.  Age-dependent response of CCAAT/enhancer binding proteins following traumatic brain injury in mice.

Authors:  Rajat Sandhir; Nancy E J Berman
Journal:  Neurochem Int       Date:  2009-10-13       Impact factor: 3.921

4.  Parvalbumin immunoreactivity and protein level are altered in the gerbil hippocampus during normal aging.

Authors:  Choong Hyun Lee; In Koo Hwang; Ki-Yeon Yoo; Jung Hoon Choi; Ok Kyu Park; In Se Lee; Moo-Ho Won
Journal:  Neurochem Res       Date:  2008-04-22       Impact factor: 3.996
  4 in total

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