| Literature DB >> 17450148 |
Fulai Jin1, Larisa Avramova, Jing Huang, Tony Hazbun.
Abstract
We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.Entities:
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Year: 2007 PMID: 17450148 PMCID: PMC2771206 DOI: 10.1038/nmeth1042
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547