A new stochastic tridimensional model of neonatal rat spinal motoneuron for investigating compartmentalization of neuronal conductances and their influence on firing.

During postnatal development spinal motoneurons play a major role in expressing basic behaviours like reflex reactions and in allowing the onset of the locomotor programme. For this purpose it is useful to clarify how various inputs are integrated at the level of the motoneuron soma to generate phasic or rhythmic firing. Although existing models of motoneurons have indicated the distributed role of certain conductances in regulating firing, it is unclear how the spatial distribution of certain currents is ultimately shaping motoneuron output. Thus, it would be helpful to build a bridge between histological and electrophysiological data. The present report is based on the construction of a 3D motoneuron model based on available parameters applicable to the neonatal spinal cord. The presented algorithm allows building up a complex, variable dendrogram which, together with the somatic and axonic compartments, enables strategic location of certain voltage or ligand gated conductances and simulation of resulting electrical behaviour. One application of the present model has been exploring the functional location of the recently reported cystic fibrosis transmembrane conductance regulator (CFTR) which controls Cl(-) homeostasis of postnatal motoneurons. The 3D model is made available for free, user friendly use via the dedicated web site http://www.mn-morphology.org.