PURPOSE: We examined the effect of electrical stimulation and lesioning of the anterior nucleus of the thalamus (ANT) on focal limbic seizures induced by intraamygdaloid kainic acid (KA) injection in a rat model. To address the mechanism underlying these anti-convulsant actions, cerebral glucose metabolism following ANT electrical stimulation and lesioning was also assessed. METHODS: Wistar rats were divided into five major groups: control, unilateral and bilateral ANT electrical stimulation, and unilateral and bilateral ANT lesioning. After KA injection, average clinical-seizure frequencies in each group were measured. Local cerebral glucose utilization (LCGU) was also measured using [(14)C] 2-deoxyglucose autoradiography in three groups: control, ANT electrical stimulation and ANT lesioning. RESULTS: Animals subjected to ANT electrical stimulation and lesioning exhibited significantly decreased mean seizure frequency and secondary generalized seizure frequency, compared with control-animals. In control-group, LCGU was markedly increased at both the limbic and corticothalamic circuits sites. While in ANT stimulation or lesioning-group, there was significant reduction in LCGU at the corticothalamic circuit sites, but not so considerable decrease at the limbic structures. CONCLUSION: ANT electrical stimulation and lesioning in the focal limbic seizure model were effective on convulsive seizures and secondary generalization, specifically with respect to the severity of these seizures.
PURPOSE: We examined the effect of electrical stimulation and lesioning of the anterior nucleus of the thalamus (ANT) on focal limbic seizures induced by intraamygdaloid kainic acid (KA) injection in a rat model. To address the mechanism underlying these anti-convulsant actions, cerebral glucose metabolism following ANT electrical stimulation and lesioning was also assessed. METHODS:Wistar rats were divided into five major groups: control, unilateral and bilateral ANT electrical stimulation, and unilateral and bilateral ANT lesioning. After KA injection, average clinical-seizure frequencies in each group were measured. Local cerebral glucose utilization (LCGU) was also measured using [(14)C] 2-deoxyglucose autoradiography in three groups: control, ANT electrical stimulation and ANT lesioning. RESULTS: Animals subjected to ANT electrical stimulation and lesioning exhibited significantly decreased mean seizure frequency and secondary generalized seizure frequency, compared with control-animals. In control-group, LCGU was markedly increased at both the limbic and corticothalamic circuits sites. While in ANT stimulation or lesioning-group, there was significant reduction in LCGU at the corticothalamic circuit sites, but not so considerable decrease at the limbic structures. CONCLUSION: ANT electrical stimulation and lesioning in the focal limbic seizure model were effective on convulsive seizures and secondary generalization, specifically with respect to the severity of these seizures.
Authors: Maisa F Miranda; Clement Hamani; Antônio-Carlos G de Almeida; Beatriz O Amorim; Carlos E Macedo; Maria José S Fernandes; José N Nobrega; Mayra C Aarão; Ana Paula Madureira; Antônio M Rodrigues; Monica L Andersen; Sergio Tufik; Luiz E Mello; Luciene Covolan Journal: Front Cell Neurosci Date: 2014-10-02 Impact factor: 5.505
Authors: Beatriz O Amorim; Luciene Covolan; Elenn Ferreira; José Geraldo Brito; Diego P Nunes; David G de Morais; José N Nobrega; Antonio M Rodrigues; Antonio Carlos G deAlmeida; Clement Hamani Journal: J Neuroinflammation Date: 2015-09-04 Impact factor: 8.322
Authors: Kim Rijkers; Véronique M P Moers-Hornikx; Roelof J Hemmes; Marlien W Aalbers; Yasin Temel; Johan S H Vles; Govert Hoogland Journal: Biomed Res Int Date: 2015-08-31 Impact factor: 3.411
Authors: Luciene Covolan; Antônio-Carlos G de Almeida; Beatriz Amorim; Clarissa Cavarsan; Maisa Ferreira Miranda; Mayra C Aarão; Ana Paula Madureira; Antônio M Rodrigues; José N Nobrega; Luiz E Mello; Clement Hamani Journal: PLoS One Date: 2014-06-03 Impact factor: 3.240