| Literature DB >> 17448514 |
Kathryn Masker1, Alicia Golden, Christian J Gaffney, Virginia Mazack, William F Schwindinger, Weizhou Zhang, Lu-Hai Wang, David J Carey, Marius Sudol.
Abstract
Transformation of chicken fibroblasts in vitro by Rous Sarcoma Virus represents a model of cancer in which a single oncogene, viral src, uniformly and rapidly transforms primary cells in culture. We experimentally surveyed the transcriptional program affected by Rous Sarcoma Virus (RSV) in primary culture of chicken embryo fibroblasts. As a control, we used cells infected with non-transforming RSV mutant td106, in which the src gene was deleted. Using Affymetrix GeneChip Chicken Genome Arrays, we report 811 genes that were modulated more than 2.5 fold in the virus transformed cells. Among these, 409 genes were induced and 402 genes were repressed by viral src. From the repertoire of modulated genes, we selected 20 genes that were robustly changed. We then validated and quantified the transcriptional changes of most of the 20 selected genes by real-time PCR. The set of strongly induced genes contains vasoactive intestinal polypeptide, MAP kinase phosphatase 2 and follistatin, among others. The set of strongly repressed genes contains TGF beta 3, TGF beta-induced gene, and deiodinase. The function of several robustly modulated genes sheds new light on the molecular mechanism of oncogenic transformation.Entities:
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Year: 2007 PMID： 17448514 PMCID： PMC1974879 DOI： 10.1016/j.virol.2007.03.026
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616