Apoptosis and blood-testis barrier during the first spermatogenic wave in the pubertal rat.

This research explores the initial assembly of the blood-testis barrier (BTB) during puberty, when a massive physiological apoptosis in the first spermatogenic wave takes place. Fragments of testis from 14- to 20-day-old rats were studied by conventional transmission electron microscopic techniques. Lanthanum hydroxide was used as an intercellular tracer. Light microscopy was used to confirm apoptotic death when paraffin-embedded sections were studied by TUNEL analysis. When the seminiferous cords reached the zygotene-pachytene spermatocyte level, they exhibited abundant apoptotic figures, whereas the remaining segments showed sporadic apoptosis. We found a BTB not yet assembled in the cords with zygotene-pachytene spermatocytes and abundant apoptosis. The observed apoptosis frequency diminished drastically when BTB was organized, as confirmed by the use of the tracer. Our conclusion is that the massive apoptosis found in the zygotene-pachytene spermatocytes between days 14 and 20 coincides with an open BTB. The absence of BTB could be one of the factors causing massive apoptosis of zygotene-pachytene spermatocytes, at least within the time span analyzed. The zygotene-pachytene spermatocytes are left exposed in an open environment instead of being isolated in the adluminal compartment to which they are destined.