Literature DB >> 17440797

A new spectrofluorimetric method for determination of trace amounts 5-hydroxytryptamine in human urine and serum.

Qian Peng1, Chongqiu Jiang.   

Abstract

A new spectrofluorimetric method was developed for the determination of trace amount of 5-hydroxytryptamine (5-HT) in human urine and serum samples. In the NaAc-HAc buffer solution of pH=5.80, 5-HT can react with formaldehyde-acetylacetone system to form a new compound which sends yellow green fluorescence at 533 nm and the enhanced fluorescence intensity is in proportion to the concentration of 5-HT. Optimum conditions for the determination of 5-HT were also investigated. The dynamic range and detection limit for the determination of 5-HT are 5.35 x 10(-7) approximately 1.07 x 10(-4) mol/L and 2.08 x 10(-7) mol/L, respectively. The developed method is simple, practical and can be successfully applied to determination of 5-HT in human urine and serum samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the 5-HT - formaldehyde-acetylacetone system have been also discussed.

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Year:  2007        PMID： 17440797     DOI： 10.1007/s10895-007-0177-1

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.525


  13 in total

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4.  The determination of 5-hydroxytryptamine, related indolealkylamines and 5-hydroxyindoleacetic acid in the bovine eye by gas chromatography-negative ion chemical ionization mass spectrometry.

Authors:  S A Best; J M Midgley; W Huang; D G Watson
Journal:  J Pharm Biomed Anal       Date:  1993 Apr-May       Impact factor: 3.935

5.  Simultaneous determination of norepinephrine, serotonin, and 5-hydroxyindole-3-acetic acid in microdialysis samples from rat brain by microbore column liquid chromatography with fluorescence detection following derivatization with benzylamine.

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Journal:  Anal Biochem       Date:  2003-01-15       Impact factor: 3.365

6.  Determination of serotonin and its precursors in human plasma by capillary electrophoresis-electrospray ionization-time-of-flight mass spectrometry.

Authors:  Zlatuse D Peterson; Milton L Lee; Steven W Graves
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2004-10-15       Impact factor: 3.205

7.  Determination of serotonin, noradrenaline, dopamine and their metabolites in rat brain extracts and microdialysis samples by column liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine.

Authors:  Takashi Yoshitake; Jan Kehr; Shimako Yoshitake; Kaoru Fujino; Hitoshi Nohta; Masatoshi Yamaguchi
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2004-08-05       Impact factor: 3.205

8.  Subminute and sensitive determination of the neurotransmitter serotonin in urine by capillary electrophoresis with laser-induced fluorescence detection.

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9.  Determination of 5-hydroxytryptamine, 5-hydroxyindoleacetic acid and tryptophan in plasma and urine by HPLC with fluorimetric detection.

Authors:  C P Bearcroft; M J Farthing; D Perrett
Journal:  Biomed Chromatogr       Date:  1995 Jan-Feb       Impact factor: 1.902

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Authors:  Y H Yi; W P Liao; X Lu
Journal:  J Chromatogr B Biomed Appl       Date:  1994-11-04
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  1 in total

1.  Two validated spectrofluorometric methods for determination of gemifloxacin mesylate in tablets and human plasma.

Authors:  Noha N Atia; Ashraf M Mahmoud; Salwa R El-Shabouri; Wesam M El-Koussi
Journal:  Int J Anal Chem       Date:  2013-05-16       Impact factor: 1.885
  1 in total

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