| Literature DB >> 17438353 |
Ming Zhou1, Hui-Jing He, Ryoji Suzuki, Ke-Xiang Liu, Osamu Tanaka, Masaki Sekiguchi, Hideaki Itoh, Katsumasa Kawahara, Hiroshi Abe.
Abstract
To understand the possible functions and subcellular localizations of sulfonylurea receptors (SURs) in cardiac muscle, polyclonal anti-SUR2A and anti-SUR2B antisera were raised. Immunoblots revealed both SUR2A and SUR2B expression in mitochondrial fractions of rat heart and other cellular fractions such as microsomes and cell membranes. Immunostaining detected ubiquitous expression of both SUR2A and SUR2B in rat heart in the atria, ventricles, interatrial and interventricular septa, and smooth muscles and endothelia of the coronary arteries. Electron microscopy revealed SUR2A immunoreactivity in the cell membrane, endoplasmic reticulum (ER), and mitochondria. SUR2B immunoreactivity was mainly localized in the mitochondria as well as in the ER and cell membrane. Thus, SUR2A and SUR2B are not only the regulatory subunits of sarcolemmal K(ATP) channels but may also function as regulatory subunits in mitochondrial K(ATP) channels and play important roles in cardioprotection.Entities:
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Year: 2007 PMID： 17438353 DOI： 10.1369/jhc.6A7104.2007
Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479