BACKGROUND: This study evaluated the in vitro efficacy of poliglecaprone 25 suture with triclosan against gram-positive and gram-negative bacteria. METHODS: Poliglecaprone 25 sutures with and without triclosan were tested for in vitro efficacy against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, methicillin-resistant Staphylococcus epidermidis (MRSE), Klebsiella pneumoniae, and Escherichia coli by a zone of inhibition assay. The suture also was tested against Escherichia coli in a colonization assay in a dynamic model simulating in vivo conditions. An in vitro triclosan diffusion assay and a sustained efficacy assay were performed by concurrent high-performance liquid chromatography and zone of inhibition assay. To assess stability, antibacterial efficacy testing was performed on samples held more than five months at elevated temperature. RESULTS: Poliglecaprone 25 suture with triclosan demonstrated significant in vitro efficacy against a range of bacteria. The suture sustained in vitro efficacy for 11 days, corresponding to the in vitro triclosan diffusion profile. CONCLUSION: Triclosan reduced in vitro colonization of poliglecaprone 25 suture by several strains of bacteria compared with untreated control sutures.
BACKGROUND: This study evaluated the in vitro efficacy of poliglecaprone 25 suture with triclosan against gram-positive and gram-negative bacteria. METHODS:Poliglecaprone 25 sutures with and without triclosan were tested for in vitro efficacy against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, methicillin-resistant Staphylococcus epidermidis (MRSE), Klebsiella pneumoniae, and Escherichia coli by a zone of inhibition assay. The suture also was tested against Escherichia coli in a colonization assay in a dynamic model simulating in vivo conditions. An in vitro triclosan diffusion assay and a sustained efficacy assay were performed by concurrent high-performance liquid chromatography and zone of inhibition assay. To assess stability, antibacterial efficacy testing was performed on samples held more than five months at elevated temperature. RESULTS:Poliglecaprone 25 suture with triclosan demonstrated significant in vitro efficacy against a range of bacteria. The suture sustained in vitro efficacy for 11 days, corresponding to the in vitro triclosan diffusion profile. CONCLUSION:Triclosan reduced in vitro colonization of poliglecaprone 25 suture by several strains of bacteria compared with untreated control sutures.
Authors: Christopher B Weldon; Jonathan H Tsui; Sahadev A Shankarappa; Vy T Nguyen; Minglin Ma; Daniel G Anderson; Daniel S Kohane Journal: J Control Release Date: 2012-05-17 Impact factor: 9.776
Authors: David Leaper; Ojan Assadian; Nils-Olaf Hubner; Andrew McBain; Thomas Barbolt; Stephen Rothenburger; Peter Wilson Journal: Int Wound J Date: 2011-08-19 Impact factor: 3.315
Authors: A Obermeier; J Schneider; P Föhr; S Wehner; K-D Kühn; A Stemberger; M Schieker; R Burgkart Journal: BMC Microbiol Date: 2015-09-24 Impact factor: 3.605
Authors: Andreas Obermeier; Jochen Schneider; Norbert Harrasser; Jutta Tübel; Heinrich Mühlhofer; Dominik Pförringer; Constantin von Deimling; Peter Foehr; Barbara Kiefel; Christina Krämer; Axel Stemberger; Matthias Schieker; Rainer Burgkart; Rüdiger von Eisenhart-Rothe Journal: PLoS One Date: 2018-01-09 Impact factor: 3.240
Authors: Manuel Bustamante Montalvo; Miguel Cainzos; Luis Prieto Carreiras; Andrea Castiñeira Piñeiro; Adrián García Iglesias; Ana Fernandez Novo; Lara María González Gómez; Ana Flores; Rita Diz Gil; Carlos Fernández Baltar Journal: Infect Prev Pract Date: 2021-06-07
Authors: Enora Laas; Cécile Poilroux; Corinne Bézu; Charles Coutant; Serge Uzan; Roman Rouzier; Elisabeth Chéreau Journal: Int J Breast Cancer Date: 2012-12-19