OBJECTIVE: To assess a possible development of antisperm antibodies (ASA), present in a high percentage of infertile patients, after organ-sparing surgery for small testicular tumours, to identify any additional immunogenic effect of this procedure compared with standard orchidectomy. PATIENTS AND METHODS: Samples of sera were assessed from 54 men who had had surgery between 2000 and 2005 for testicular tumour; the men were divided into two groups, i.e. group A (23) had had organ-sparing tumour resection and group B (31) had had inguinal orchidectomy. Other possible causes of ASA besides testicular tumour were excluded in all patients. The blood samples were obtained during follow-up visits and the circulating ASA in serum determined using an enzyme-linked immunosorbent assay. RESULTS: The mean (range) tumour diameter was statistically significantly greater (P < 0.03) in group B, at 33.6 (2-130) mm, than in group A, at 12 (2-30) mm. There were significantly more stromal tumours in group A than group B (P < 0.02). Most importantly, the mean (range) ASA levels were not statistically significantly (P > 0.3) higher in group A, at 29 (15-59) U/mL, than in group B, at 24.8 (12-39) U/mL. There was also no statistically significant correlation between ASA levels and clinical stage, length of follow-up after therapy, patient age, tumour size and type of histology. CONCLUSION: From these data, organ-sparing surgery does not lead to greater ASA levels than orchidectomy and patients are therefore at no greater risk of developing an autoimmune infertility.
OBJECTIVE: To assess a possible development of antisperm antibodies (ASA), present in a high percentage of infertilepatients, after organ-sparing surgery for small testicular tumours, to identify any additional immunogenic effect of this procedure compared with standard orchidectomy. PATIENTS AND METHODS: Samples of sera were assessed from 54 men who had had surgery between 2000 and 2005 for testicular tumour; the men were divided into two groups, i.e. group A (23) had had organ-sparing tumour resection and group B (31) had had inguinal orchidectomy. Other possible causes of ASA besides testicular tumour were excluded in all patients. The blood samples were obtained during follow-up visits and the circulating ASA in serum determined using an enzyme-linked immunosorbent assay. RESULTS: The mean (range) tumour diameter was statistically significantly greater (P < 0.03) in group B, at 33.6 (2-130) mm, than in group A, at 12 (2-30) mm. There were significantly more stromal tumours in group A than group B (P < 0.02). Most importantly, the mean (range) ASA levels were not statistically significantly (P > 0.3) higher in group A, at 29 (15-59) U/mL, than in group B, at 24.8 (12-39) U/mL. There was also no statistically significant correlation between ASA levels and clinical stage, length of follow-up after therapy, patient age, tumour size and type of histology. CONCLUSION: From these data, organ-sparing surgery does not lead to greater ASA levels than orchidectomy and patients are therefore at no greater risk of developing an autoimmune infertility.