OBJECTIVE: To establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection. METHOD: Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm. RESULT: The mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively. CONCLUSION: The method was simple, rapid, accurate and specific and suitable for quality control of the product.
OBJECTIVE: To establish a high performance liquid chromatography (HPLC) method for determination of matrine, sophoridine and oxymatrine in Compound Kushen Injection. METHOD: Alltima amino column was used with acetonitrile-3% phosphoric acid-ethyl alcohol (80:10:10) as the mobile phase and the detection wavelength was at 220 nm. RESULT: The mean recovery of matrine, sophoridine and oxymatrine in Compound Kushen Injection was 99.51% (RSD 1. 58%), 99.24% (RSD 1.44%) and 100.22% (RSD 1.85%), respectively. CONCLUSION: The method was simple, rapid, accurate and specific and suitable for quality control of the product.