| Literature DB >> 17428464 |
Zsuzsanna E Toth1, Tal Shahar, Ronen Leker, Ildiko Szalayova, András Bratincsák, Sharon Key, Anna Lonyai, Krisztián Németh, Eva Mezey.
Abstract
The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in the literature regarding the possible fate choices of stem cells. Here we describe a very sensitive technique for visualization of GFP. Using it we can detect about 90% of cells of donor origin while we could only see about 50% of these cells when we employ the methods that are in general use in other laboratories. In addition, we provide evidence that some cells permanently silence GFP expression. In the case of the progeny of bone marrow stem cells, it appears that the more distantly related they are to their precursors, the more likely it is that they will turn off the lineage marker.Entities:
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Year: 2007 PMID： 17428464 PMCID： PMC1950935 DOI： 10.1016/j.yexcr.2007.02.024
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905