| Literature DB >> 17417645 |
Liora Haim1, Gadi Zipor, Stella Aronov, Jeffrey E Gerst.
Abstract
mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3' untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP fused with GFP, we demonstrate the localization of endogenous mRNAs (ASH1, SRO7, PEX3 and OXA1) in living yeast (Saccharomyces cerevisiae).Entities:
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Year: 2007 PMID： 17417645 DOI： 10.1038/nmeth1040
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547