Literature DB >> 1741276

Purification and properties of the MboII, a class-IIS restriction endonuclease.

M Sektas1, T Kaczorowski, A J Podhajska.   

Abstract

After five purification steps a homogeneous preparation of endonuclease MboII was obtained, and several properties of the enzyme were determined. MboII is a monomer, with Mr under native and denaturing conditions being 47-49 x 10(3) Da. Endonuclease MboII is a basic protein (pI 8.3) which remains active when Mg2+ is replaced by Mn2+, Co2+, Ca2+, or Fe2+. MboII exhibits a star activity in the presence of some of the following reagents or ions: DMSO, glycerol, ethanol (and Co2+ or Mn2+ at pH 6). MboII does not bend DNA and is heat sensitive, losing activity after 15 min at 50 degrees C.

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Year:  1992        PMID： 1741276      PMCID： PMC310404          DOI： 10.1093/nar/20.3.433

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  35 in total

1.  A method for determining the sedimentation behavior of enzymes: application to protein mixtures.

Authors:  R G MARTIN; B N AMES
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

Review 2.  Class-IIS restriction enzymes--a review.

Authors:  W Szybalski; S C Kim; N Hasan; A J Podhajska
Journal:  Gene       Date:  1991-04       Impact factor: 3.688

3.  Alteration of the specificity of PvuII restriction endonuclease.

Authors:  M Nasri; D Thomas
Journal:  Nucleic Acids Res       Date:  1987-10-12       Impact factor: 16.971

Review 4.  The EcoR V restriction endonuclease.

Authors:  P A Luke; S A McCallum; S E Halford
Journal:  Gene Amplif Anal       Date:  1987

5.  Formation of MboII vectors and cassettes using asymmetric MboII linkers.

Authors:  R B Gayle; E A Auger; G R Gough; P T Gilham; G N Bennett
Journal:  Gene       Date:  1987       Impact factor: 3.688

6.  Cloning of two type II methylase genes that recognise asymmetric nucleotide sequences: FokI and HgaI.

Authors:  D Nwankwo; G Wilson
Journal:  Mol Gen Genet       Date:  1987-10

7.  Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.

Authors:  A C Boyd; I G Charles; J W Keyte; W J Brammar
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

8.  Cleaving DNA at any predetermined site with adapter-primers and class-IIS restriction enzymes.

Authors:  S C Kim; A J Podhajska; W Szybalski
Journal:  Science       Date:  1988-04-22       Impact factor: 47.728

9.  Cross-linking of bromodeoxyuridine-substituted oligonucleotides to the EcoRI and EcoRV restriction endonucleases.

Authors:  H Wolfes; A Fliess; F Winkler; A Pingoud
Journal:  Eur J Biochem       Date:  1986-09-01

10.  Tet repressor binding induced curvature of tet operator DNA.

Authors:  K Tovar; W Hillen
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971
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  6 in total

1.  The FokI methyltransferase from Flavobacterium okeanokoites. Purification and characterization of the enzyme and its truncated derivatives.

Authors:  T Kaczorowski; M Sektas; P Skowron; A J Podhajska
Journal:  Mol Biotechnol       Date:  1999-11       Impact factor: 2.695

2.  FokI dimerization is required for DNA cleavage.

Authors:  J Bitinaite; D A Wah; A K Aggarwal; I Schildkraut
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

3.  Role of the deletion of polymorphism of the angiotensin converting enzyme gene in the progression and therapeutic responsiveness of IgA nephropathy.

Authors:  H Yoshida; T Mitarai; T Kawamura; T Kitajima; Y Miyazaki; R Nagasawa; Y Kawaguchi; H Kubo; I Ichikawa; O Sakai
Journal:  J Clin Invest       Date:  1995-11       Impact factor: 14.808

4.  Genetic organization and molecular analysis of the EcoVIII restriction-modification system of Escherichia coli E1585-68 and its comparison with isospecific homologs.

Authors:  Iwona Mruk; Tadeusz Kaczorowski
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

5.  Restriction endonuclease BpuJI specific for the 5'-CCCGT sequence is related to the archaeal Holliday junction resolvase family.

Authors:  Rasa Sukackaite; Arunas Lagunavicius; Kornelijus Stankevicius; Claus Urbanke; Ceslovas Venclovas; Virginijus Siksnys
Journal:  Nucleic Acids Res       Date:  2007-03-28       Impact factor: 16.971

6.  The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases.

Authors:  Hua Wei; Caitlin Therrien; Aine Blanchard; Shengxi Guan; Zhenyu Zhu
Journal:  Nucleic Acids Res       Date:  2008-04-15       Impact factor: 16.971
  6 in total

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