BACKGROUND: Perforin is a membrane-disrupting protein that allows the entry of granzymes into a target cell inducing degradation of target substances in the cytoplasm and nucleus thus leading to programmed cell death or apoptosis. Recent work demonstrated a possible involvement of perforin mediated cytotoxicity in immunopathogenesis of psoriasis. OBJECTIVES: To investigate a difference in systemic (peripheral blood) and local (lesions) expression and distribution of perforin in psoriatic patients with severe and mild disease. METHODS: Flow cytometry was used for simultaneous detection of intracellular (perforin) and cell surface antigens in peripheral blood lymphocytes. The expression of perforin in skin lesions was evaluated by immunohistochemistry. RESULTS: Significant increase of perforin expression in T lymphocytes, especially cytotoxic CD8+ cells was found in severe psoriasis compared to mild disease (p<0.01 and p<0.05, respectively). There was also an increase of CD56+P+ NK cells (p<0.05) in severe compared to mild psoriasis. The psoriatic plaque of both, severe and mild disease were abundant with perforin showing no significant difference on local level. CONCLUSION: Based on our results we suggest the association between perforin expression and disease severity.
BACKGROUND: Perforin is a membrane-disrupting protein that allows the entry of granzymes into a target cell inducing degradation of target substances in the cytoplasm and nucleus thus leading to programmed cell death or apoptosis. Recent work demonstrated a possible involvement of perforin mediated cytotoxicity in immunopathogenesis of psoriasis. OBJECTIVES: To investigate a difference in systemic (peripheral blood) and local (lesions) expression and distribution of perforin in psoriaticpatients with severe and mild disease. METHODS: Flow cytometry was used for simultaneous detection of intracellular (perforin) and cell surface antigens in peripheral blood lymphocytes. The expression of perforin in skin lesions was evaluated by immunohistochemistry. RESULTS: Significant increase of perforin expression in T lymphocytes, especially cytotoxic CD8+ cells was found in severe psoriasis compared to mild disease (p<0.01 and p<0.05, respectively). There was also an increase of CD56+P+ NK cells (p<0.05) in severe compared to mild psoriasis. The psoriatic plaque of both, severe and mild disease were abundant with perforin showing no significant difference on local level. CONCLUSION: Based on our results we suggest the association between perforin expression and disease severity.
Authors: Marcel B M Teunissen; Nataliya G Yeremenko; Dominique L P Baeten; Saskia Chielie; Phyllis I Spuls; Menno A de Rie; Olivier Lantz; Pieter C M Res Journal: J Invest Dermatol Date: 2014-06-18 Impact factor: 8.551
Authors: Fernanda O Novais; Lucas P Carvalho; Sara Passos; David S Roos; Edgar M Carvalho; Phillip Scott; Daniel P Beiting Journal: J Invest Dermatol Date: 2014-07-18 Impact factor: 8.551
Authors: Fernanda O Novais; Augusto M Carvalho; Megan L Clark; Lucas P Carvalho; Daniel P Beiting; Igor E Brodsky; Edgar M Carvalho; Phillip Scott Journal: PLoS Pathog Date: 2017-02-13 Impact factor: 6.823