| Literature DB >> 17408621 |
I Howard Cukier1, Yun Li, Jonathan M Lee.
Abstract
Substantial actin remodelling occurs prior to mitosis as cells alter their shape in preparation for cytokinesis. In mammalian cells, mitosis is initiated by a heterodimer of cyclin B1 and the cyclin dependent kinase 1 (Cdk1) serine/threonine kinase. In this report. we show that human cyclin B1 binds the actin cross-linking protein Filamin-A (FLNa). The proteins co-immunoprecipitate and co-localize in mitotic human cells. We find that cyclin B1/Cdk1 can phosphorylate FLNa in vitro and reduce its ability to gelate actin. We have also identified serine 1436 as one FLNa residue phosphorylated by cyclin B1/Cdk1 in vitro. Our results suggest a role for cyclin B1/Cdk1 in FLNa-dependent actin remodelling.Entities:
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Year: 2007 PMID： 17408621 DOI： 10.1016/j.febslet.2007.03.041
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124