| Literature DB >> 1740755 |
O Grankvist1, U Bredberg-Rådèn, A Gustafsson, J Albert, P Albino, P A Andreasson, A Nauclér, G Biberfeld, G Wadell.
Abstract
A two-step polymerase chain reaction (PCR), with four double (nested) primer pairs, used for the detection of HIV-2 in clinical samples is described. With these four nested primer pairs we could detect HIV-2 DNA in 17 of 17 virus isolates and in blood mononuclear cell samples from 31 of 37 (83.7%) seropositive individuals after ethidium bromide staining of the amplified DNA. The nested primer PCR was also compared with a single primer pair-based PCR followed by hybridization. The sensitivities of the two methods were almost equal, but the nested primer PCR offered obvious technical advantages.Entities:
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Year: 1992 PMID： 1740755
Source DB: PubMed Journal: J Acquir Immune Defic Syndr (1988) ISSN： 0894-9255