Literature DB >> 17406529

Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism.

Norma J Greenfield1.   

Abstract

Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include determination of the free energy of folding of a protein, the effects of mutations on protein stability and the estimation of binding constants for the interactions of proteins with other proteins, DNA or ligands, such as substrates or inhibitors. The experiments require 2-5 h.

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Year:  2006        PMID： 17406529      PMCID： PMC2728349          DOI： 10.1038/nprot.2006.229

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  39 in total

1.  Theory of weak molecular complexes. III. Observation equations for multiple equilibria and an application to protein charge-transfer titrations.

Authors:  D A Deranleau
Journal:  J Am Chem Soc       Date:  1975-03-05       Impact factor: 15.419

2.  Induced circular dichroism by chiral molecular interaction.

Authors:  Stig Allenmark
Journal:  Chirality       Date:  2003-05-15       Impact factor: 2.437

Review 3.  Circular dichroism analysis for protein-protein interactions.

Authors:  Norma J Greenfield
Journal:  Methods Mol Biol       Date:  2004

Review 4.  Analysis of circular dichroism data.

Authors:  Norma J Greenfield
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

5.  Using circular dichroism spectra to estimate protein secondary structure.

Authors:  Norma J Greenfield
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice.

Authors:  J D McGhee; P H von Hippel
Journal:  J Mol Biol       Date:  1974-06-25       Impact factor: 5.469

7.  Spectroscopic identification of binding modes of anthracene probes and DNA sequence recognition.

Authors:  Willy B Tan; Akhilesh Bhambhani; Michael R Duff; Alison Rodger; Challa V Kumar
Journal:  Photochem Photobiol       Date:  2006 Jan-Feb       Impact factor: 3.421

8.  Mutations in the N- and D-helices of the N-domain of troponin C affect the C-domain and regulatory function.

Authors:  L Smith; N J Greenfield; S E Hitchcock-DeGregori
Journal:  Biophys J       Date:  1999-01       Impact factor: 4.033

9.  The effects of deletion of the amino-terminal helix on troponin C function and stability.

Authors:  L Smith; N J Greenfield; S E Hitchcock-DeGregori
Journal:  J Biol Chem       Date:  1994-04-01       Impact factor: 5.157

10.  Protein stability: urea-induced versus guanidine-induced unfolding of metmyoglobin.

Authors:  R Gupta; S Yadav; F Ahmad
Journal:  Biochemistry       Date:  1996-09-10       Impact factor: 3.162
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  34 in total

1.  Using circular dichroism collected as a function of temperature to determine the thermodynamics of protein unfolding and binding interactions.

Authors:  Norma J Greenfield
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

2.  Analysis of the kinetics of folding of proteins and peptides using circular dichroism.

Authors:  Norma J Greenfield
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

3.  Using circular dichroism spectra to estimate protein secondary structure.

Authors:  Norma J Greenfield
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

4.  Purification, Biochemical Characterization, and Amino Acid Sequence of a Novel Type of Lectin from Aplysia dactylomela Eggs with Antibacterial/Antibiofilm Potential.

Authors:  Rômulo Farias Carneiro; Renato Cézar Farias Torres; Renata Pinheiro Chaves; Mayron Alves de Vasconcelos; Bruno Lopes de Sousa; André Castelo Rodrigues Goveia; Francisco Vassiliepe Arruda; Maria Nágila Carneiro Matos; Helena Matthews-Cascon; Valder Nogueira Freire; Edson Holanda Teixeira; Celso Shiniti Nagano; Alexandre Holanda Sampaio
Journal:  Mar Biotechnol (NY)       Date:  2017-02-01       Impact factor: 3.619

5.  Netropsin binding in five duplex-dimer DNA constructs as a function of size and distance between binding sites: circular dichroism and absorption spectroscopy.

Authors:  Lavanya Premvardhan; Jean-Claude Maurizot
Journal:  Eur Biophys J       Date:  2009-10-28       Impact factor: 1.733

6.  The Interplay between Molten Globules and Heme Disassociation Defines Human Hemoglobin Disassembly.

Authors:  Premila P Samuel; Mark A White; William C Ou; David A Case; George N Phillips; John S Olson
Journal:  Biophys J       Date:  2020-02-04       Impact factor: 4.033

7.  Assessing and Improving Protein Sample Quality.

Authors:  Bertrand Raynal; Sébastien Brûlé; Stephan Uebel; Stefan H Knauer
Journal:  Methods Mol Biol       Date:  2021

8.  APOL1 variants change C-terminal conformational dynamics and binding to SNARE protein VAMP8.

Authors:  Sethu M Madhavan; John F O'Toole; Martha Konieczkowski; Laura Barisoni; David B Thomas; Santhi Ganesan; Leslie A Bruggeman; Matthias Buck; John R Sedor
Journal:  JCI Insight       Date:  2017-07-20

9.  Structure of the N terminus of a nonmuscle alpha-tropomyosin in complex with the C terminus: implications for actin binding.

Authors:  Norma J Greenfield; Lucy Kotlyanskaya; Sarah E Hitchcock-DeGregori
Journal:  Biochemistry       Date:  2009-02-17       Impact factor: 3.162

10.  Rapid and efficient purification of RNA-binding proteins: application to HIV-1 Rev.

Authors:  Marco Marenchino; David W Armbruster; Mirko Hennig
Journal:  Protein Expr Purif       Date:  2008-09-25       Impact factor: 1.650
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