Literature DB >> 17406452

A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction.

Ngan F Huang1, Richard E Sievers, Jennifer S Park, Qizhi Fang, Song Li, Randall J Lee.   

Abstract

We have developed a robust rat model of myocardial infarction (MI). Here we describe the step-by-step protocol for creating an ischemia-reperfusion rat model of MI. We also describe how to deliver therapeutic injections of mesenchymal stem cells (MSCs) together with fibrin, to show an application of this model. In addition, to confirm the presence of fibrin and cells in the infarct, visualization of MSCs and fibrin by histological techniques are also described. The ischemia-reperfusion MI model can be modified and generalized for use with various injectable polymers, cell types, drugs, DNA and combinations thereof. The model can be created in 7 days or less, depending on the timing of therapeutic intervention.

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Year:  2006        PMID： 17406452     DOI： 10.1038/nprot.2006.188

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  25 in total

1.  Approach to assessing myocardial perfusion in rats using static [13N]-ammonia images and a small-animal PET.

Authors:  Juan José Vaquero; Dong-Wei Gao; Carmen García-Villaba; Stephen Bacharach; Henry Vanbrocklin; Qizhi Fang; Manuel Desco; Randall Lee; Michael Dae
Journal:  Mol Imaging Biol       Date:  2012-10       Impact factor: 3.488

2.  Bioreducible polymer-transfected skeletal myoblasts for VEGF delivery to acutely ischemic myocardium.

Authors:  Arlo N McGinn; Hye Yeong Nam; Mei Ou; Norman Hu; Catherine M Straub; James W Yockman; David A Bull; Sung Wan Kim
Journal:  Biomaterials       Date:  2010-11-05       Impact factor: 12.479

3.  Biophysical and chemical effects of fibrin on mesenchymal stromal cell gene expression.

Authors:  Ngan F Huang; Julia Chu; Randall J Lee; Song Li
Journal:  Acta Biomater       Date:  2010-05-26       Impact factor: 8.947

Review 4.  Model systems for cardiovascular regenerative biology.

Authors:  Jessica C Garbern; Christine L Mummery; Richard T Lee
Journal:  Cold Spring Harb Perspect Med       Date:  2013-04-01       Impact factor: 6.915

5.  Development of Injectable Amniotic Membrane Matrix for Postmyocardial Infarction Tissue Repair.

Authors:  Jeffrey J D Henry; Lawrence Delrosario; Jun Fang; Sze Yue Wong; Qizhi Fang; Richard Sievers; Surya Kotha; Aijun Wang; Diana Farmer; Praneeth Janaswamy; Randall J Lee; Song Li
Journal:  Adv Healthc Mater       Date:  2019-11-28       Impact factor: 9.933

6.  Catheter-deliverable hydrogel derived from decellularized ventricular extracellular matrix increases endogenous cardiomyocytes and preserves cardiac function post-myocardial infarction.

Authors:  Jennifer M Singelyn; Priya Sundaramurthy; Todd D Johnson; Pamela J Schup-Magoffin; Diane P Hu; Denver M Faulk; Jean Wang; Kristine M Mayle; Kendra Bartels; Michael Salvatore; Adam M Kinsey; Anthony N Demaria; Nabil Dib; Karen L Christman
Journal:  J Am Coll Cardiol       Date:  2012-02-21       Impact factor: 24.094

7.  Surgical models for cardiac regeneration in neonatal mice.

Authors:  Ahmed I Mahmoud; Enzo R Porrello; Wataru Kimura; Eric N Olson; Hesham A Sadek
Journal:  Nat Protoc       Date:  2014-01-16       Impact factor: 13.491

8.  Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering.

Authors:  Jennifer M Singelyn; Jessica A DeQuach; Sonya B Seif-Naraghi; Robert B Littlefield; Pamela J Schup-Magoffin; Karen L Christman
Journal:  Biomaterials       Date:  2009-07-15       Impact factor: 12.479

9.  Targeted in vivo extracellular matrix formation promotes neovascularization in a rodent model of myocardial infarction.

Authors:  Shirley S Mihardja; Dongwei Gao; Richard E Sievers; Qizhi Fang; Jinjin Feng; Jianming Wang; Henry F Vanbrocklin; James W Larrick; Manley Huang; Michael Dae; Randall J Lee
Journal:  PLoS One       Date:  2010-04-28       Impact factor: 3.240

10.  Micro-structurally detailed model of a therapeutic hydrogel injectate in a rat biventricular cardiac geometry for computational simulations.

Authors:  Mazin S Sirry; Neil H Davies; Karen Kadner; Laura Dubuis; Muhammad G Saleh; Ernesta M Meintjes; Bruce S Spottiswoode; Peter Zilla; Thomas Franz
Journal:  Comput Methods Biomech Biomed Engin       Date:  2013-05-20       Impact factor: 1.763
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