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Literature DB >> 17406450

Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol.

Brittney-Shea Herbert¹, Amelia E Hochreiter, Woodring E Wright, Jerry W Shay.

Abstract

The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).

Mesh：

Substances：
DNA Primers
Telomerase

Year: 2006 PMID： 17406450 DOI： 10.1038/nprot.2006.239

Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491

Keyword Cloud
Cited

82 in total

1. HMGB1 gene knockout in mouse embryonic fibroblasts results in reduced telomerase activity and telomere dysfunction.

Authors: Eva Polanská; Zuzana Dobšáková; Martina Dvořáčková; Jiří Fajkus; Michal Štros
Journal: Chromosoma Date: 2012-04-28 Impact factor: 4.316

2. Inter-telomeric recombination is present in telomerase-positive human cells.

Authors: Margit Dlaska; Patrick Schöffski; Oliver E Bechter
Journal: Cell Cycle Date: 2013-06-06 Impact factor: 4.534

3. Prospective identification, isolation, and profiling of a telomerase-expressing subpopulation of human neural stem cells, using sox2 enhancer-directed fluorescence-activated cell sorting.

Authors: Su Wang; Devin Chandler-Militello; Gang Lu; Neeta S Roy; Alex Zielke; Romane Auvergne; Nancy Stanwood; Daniel Geschwind; Giovanni Coppola; Silvia K Nicolis; Fraser J Sim; Steven A Goldman
Journal: J Neurosci Date: 2010-11-03 Impact factor: 6.167

4. Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance.

Authors: Mengfan Tang; Yujing Li; Yi Zhang; Yuxi Chen; Wenjun Huang; Dan Wang; Arthur J Zaug; Dan Liu; Yong Zhao; Thomas R Cech; Wenbin Ma; Zhou Songyang
Journal: J Cell Sci Date: 2014-11-21 Impact factor: 5.285

5. A PCR-free voltammetric telomerase activity assay using a substrate primer on a gold electrode and DNA-triggered capture of gold nanoparticles.

Authors: Fanyu Meng; Yuanyuan Xu; Wenfei Dong; Yuguo Tang; Peng Miao
Journal: Mikrochim Acta Date: 2018-08-01 Impact factor: 5.833

10. Herpesvirus telomerase RNA(vTR)-dependent lymphoma formation does not require interaction of vTR with telomerase reverse transcriptase (TERT).

Authors: Benedikt B Kaufer; Sascha Trapp; Keith W Jarosinski; Nikolaus Osterrieder
Journal: PLoS Pathog Date: 2010-08-26 Impact factor: 6.823

Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol.

1. HMGB1 gene knockout in mouse embryonic fibroblasts results in reduced telomerase activity and telomere dysfunction.

2. Inter-telomeric recombination is present in telomerase-positive human cells.

3. Prospective identification, isolation, and profiling of a telomerase-expressing subpopulation of human neural stem cells, using sox2 enhancer-directed fluorescence-activated cell sorting.

4. Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance.

5. A PCR-free voltammetric telomerase activity assay using a substrate primer on a gold electrode and DNA-triggered capture of gold nanoparticles.

6. Primary culture and immortalization of human fallopian tube secretory epithelial cells.

7. Cyst Fluid Telomerase Activity Predicts the Histologic Grade of Cystic Neoplasms of the Pancreas.

8. Analysis of 41 cancer cell lines reveals excessive allelic loss and novel mutations in the SIRT1 gene.

9. DNAzyme-based probes for telomerase detection in early-stage cancer diagnosis.

10. Herpesvirus telomerase RNA(vTR)-dependent lymphoma formation does not require interaction of vTR with telomerase reverse transcriptase (TERT).