Literature DB >> 17406404

Expanded-bed adsorption immobilized-metal affinity chromatography.

Berend Tolner1, Lisa Smith, Richard H J Begent, Kerry A Chester.   

Abstract

The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure is any crude feedstock that contains a histidine (His)-tagged target protein. The protocol is exemplified using unclarified broth from Pichia pastoris fermentation as feedstock. The protocol can be used for laboratory studies or as part of a process for production of recombinant biotherapeutics to standards of good manufacturing practice. It takes approximately 5 h to purify proteins from 10 liters of feedstock and a further 5-6 h to sterilize and regenerate the column.

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Year:  2006        PMID： 17406404     DOI： 10.1038/nprot.2006.127

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  6 in total

1.  Development of a method to extract and purify target compounds from medicinal plants in a single step: online hyphenation of expanded bed adsorption chromatography and countercurrent chromatography.

Authors:  Yang Li; Nan Wang; Min Zhang; Yoichiro Ito; Hongyang Zhang; Yuerong Wang; Xin Guo; Ping Hu
Journal:  Anal Chem       Date:  2014-03-13       Impact factor: 6.986

Review 2.  Imaging in targeted delivery of therapy to cancer.

Authors:  Gairin Dancey; Richard H Begent; Tim Meyer
Journal:  Target Oncol       Date:  2009-10-08       Impact factor: 4.493

3.  Engineering a single-chain Fv antibody to alpha v beta 6 integrin using the specificity-determining loop of a foot-and-mouth disease virus.

Authors:  Heide Kogelberg; Berend Tolner; Gareth J Thomas; Danielle Di Cara; Shane Minogue; Bala Ramesh; Serena Sodha; Dan Marsh; Mark W Lowdell; Tim Meyer; Richard H J Begent; Ian Hart; John F Marshall; Kerry Chester
Journal:  J Mol Biol       Date:  2008-07-16       Impact factor: 5.469

4.  Generation and characterization of a diabody targeting the αvβ6 integrin.

Authors:  Heide Kogelberg; Enrique Miranda; Jerome Burnet; David Ellison; Berend Tolner; Julie Foster; Carmen Picón; Gareth J Thomas; Tim Meyer; John F Marshall; Stephen J Mather; Kerry Chester
Journal:  PLoS One       Date:  2013-09-04       Impact factor: 3.240

5.  IMAC capture of recombinant protein from unclarified mammalian cell feed streams.

Authors:  Alexander Kinna; Berend Tolner; Enrique Miranda Rota; Nigel Titchener-Hooker; Darren Nesbeth; Kerry Chester
Journal:  Biotechnol Bioeng       Date:  2015-09-03       Impact factor: 4.530

6.  The use of a surface active agent in the protection of a fusion protein during bioprocessing.

Authors:  Peter Blas; Berend Tolner; John Ward; Kerry Chester; Mike Hoare
Journal:  Biotechnol Bioeng       Date:  2018-09-08       Impact factor: 4.530
  6 in total

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