Literature DB >> 1740350

Association of recombinant soluble IL-6-signal transducer, gp130, with a complex of IL 6 and soluble IL-6 receptor, and establishment of an ELISA for soluble gp130.

K Yasukawa1, K Futatsugi, T Saito, H Yawata, M Narazaki, H Suzuki, T Taga, T Kishimoto.   

Abstract

IL-6 mediates its pleiotropic functions through two membrane proteins, a ligand-binding molecule (IL-6 receptor, IL-6R) and a non-ligand-binding signal transducer (gp130). Starting with a previously isolated cDNA clone encoding human gp130, recombinant soluble gp130 (sgp130) lacking the transmembrane and cytoplasmic regions was expressed in COS7 cells or CHO cells. sgp130 could associate with a complex of IL-6 and soluble IL-6R (sIL-6R), also lacking transmembrane and cytoplasmic regions. This indicated that extracellular region of gp130 was responsible for the association with IL-6R which was occupied by IL-6. An enzyme-linked immunosorbent assay (ELISA) for the quantitation of sgp130 was established, which was based on the interaction of sgp130 with the complex of IL-6 and sIL-6R and could detect sgp130 as low as 1 ng/ml.

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Year:  1992        PMID： 1740350     DOI： 10.1016/0165-2478(92)90138-e

Source DB:  PubMed          Journal:  Immunol Lett        ISSN： 0165-2478            Impact factor:   3.685


  18 in total

1.  Functional inhibition of hematopoietic and neurotrophic cytokines by blocking the interleukin 6 signal transducer gp130.

Authors:  T Taga; M Narazaki; K Yasukawa; T Saito; D Miki; M Hamaguchi; S Davis; M Shoyab; G D Yancopoulos; T Kishimoto
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-15       Impact factor: 11.205

2.  Fetal liver development requires a paracrine action of oncostatin M through the gp130 signal transducer.

Authors:  A Kamiya; T Kinoshita; Y Ito; T Matsui; Y Morikawa; E Senba; K Nakashima; T Taga; K Yoshida; T Kishimoto; A Miyajima
Journal:  EMBO J       Date:  1999-04-15       Impact factor: 11.598

3.  A form of circulating interleukin-6 receptor component soluble gp130 as a potential interleukin-6 inhibitor in inflammatory bowel disease.

Authors:  K Mitsuyama; N Tomiyasu; A Suzuki; K Takaki; H Takedatsu; J Masuda; H Yamasaki; S Matsumoto; O Tsuruta; A Toyonaga; M Sata
Journal:  Clin Exp Immunol       Date:  2006-01       Impact factor: 4.330

Review 4.  Interleukin-6: structure-function relationships.

Authors:  R J Simpson; A Hammacher; D K Smith; J M Matthews; L D Ward
Journal:  Protein Sci       Date:  1997-05       Impact factor: 6.725

5.  Ligand-specific utilization of the extracellular membrane-proximal region of the gp130-related signalling receptors.

Authors:  A Hammacher; J Wijdenes; D J Hilton; N A Nicola; R J Simpson; J E Layton
Journal:  Biochem J       Date:  2000-01-01       Impact factor: 3.857

6.  Soluble interleukin-6 receptors in inflammatory bowel disease: relation to circulating interleukin-6.

Authors:  K Mitsuyama; A Toyonaga; E Sasaki; O Ishida; H Ikeda; O Tsuruta; K Harada; H Tateishi; T Nishiyama; K Tanikawa
Journal:  Gut       Date:  1995-01       Impact factor: 23.059

7.  gp130 and c-Kit signalings synergize for ex vivo expansion of human primitive hemopoietic progenitor cells.

Authors:  X Sui; K Tsuji; R Tanaka; S Tajima; K Muraoka; Y Ebihara; K Ikebuchi; K Yasukawa; T Taga; T Kishimoto
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

8.  Serum levels of IL-6 type cytokines and soluble IL-6 receptors in active B-cell chronic lymphocytic leukemia and in cladribine induced remission.

Authors:  T Robak; A Wierzbowska; M Błasińska-Morawiec; A Korycka; J Z Błoński
Journal:  Mediators Inflamm       Date:  1999       Impact factor: 4.711

9.  Generation of interleukin-6 receptor antagonists by molecular-modeling guided mutagenesis of residues important for gp130 activation.

Authors:  R Savino; A Lahm; A L Salvati; L Ciapponi; E Sporeno; S Altamura; G Paonessa; C Toniatti; G Ciliberto
Journal:  EMBO J       Date:  1994-03-15       Impact factor: 11.598

10.  Structure-function analysis of human IL-6 receptor: dissociation of amino acid residues required for IL-6-binding and for IL-6 signal transduction through gp130.

Authors:  H Yawata; K Yasukawa; S Natsuka; M Murakami; K Yamasaki; M Hibi; T Taga; T Kishimoto
Journal:  EMBO J       Date:  1993-04       Impact factor: 11.598
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