OBJECTIVE: The purpose of this study was to investigate vascular reactivity in heterozygous and homozygous offspring with a genetic predisposition for hypertension after postnatal cross-fostering to mothers with the opposite genetic inheritance of the NOS3 knockout allele. STUDY DESIGN: Homozygous NOS3 knockout (C57BL/6J-NOS3(-/-KO)) and wild-type mice (NOS3(+/+WT)) were bred to obtain heterozygous litters with a paternally derived (NOS3(+/-pat)) or maternally derived (NOS3(+/-mat)) knockout allele. After delivery, heterozygous and homozygous litters were cross-fostered to a mother with the opposite NOS3 gene status. Carotid arteries were placed in a wire myograph for isometric tension recording with the use of contractile and relaxant agents. Statistical analysis with 1-way analysis of variance and Neuman-Keuls post-hoc testing was performed. RESULTS: Increased sensitivity to phenylephrine and absent relaxation to acetylcholine in NOS3(+/-mat) was reversed with cross-fostering, and vasorelaxation to isoproterenol was increased. Contraction to calcium was increased in the cross-fostered paternally derived and wild-type litters. CONCLUSION: Postnatal interventions may alter the adult vascular profile favorably that is the result of an abnormal intrauterine environment.
OBJECTIVE: The purpose of this study was to investigate vascular reactivity in heterozygous and homozygous offspring with a genetic predisposition for hypertension after postnatal cross-fostering to mothers with the opposite genetic inheritance of the NOS3 knockout allele. STUDY DESIGN: Homozygous NOS3 knockout (C57BL/6J-NOS3(-/-KO)) and wild-type mice (NOS3(+/+WT)) were bred to obtain heterozygous litters with a paternally derived (NOS3(+/-pat)) or maternally derived (NOS3(+/-mat)) knockout allele. After delivery, heterozygous and homozygous litters were cross-fostered to a mother with the opposite NOS3 gene status. Carotid arteries were placed in a wire myograph for isometric tension recording with the use of contractile and relaxant agents. Statistical analysis with 1-way analysis of variance and Neuman-Keuls post-hoc testing was performed. RESULTS: Increased sensitivity to phenylephrine and absent relaxation to acetylcholine in NOS3(+/-mat) was reversed with cross-fostering, and vasorelaxation to isoproterenol was increased. Contraction to calcium was increased in the cross-fostered paternally derived and wild-type litters. CONCLUSION: Postnatal interventions may alter the adult vascular profile favorably that is the result of an abnormal intrauterine environment.
Authors: S Rotmensch; M Liberati; J S Luo; H J Kliman; Y Gollin; U Bellati; J C Hobbins; J A Copel Journal: Am J Obstet Gynecol Date: 1994-11 Impact factor: 8.661
Authors: Monica Longo; Venu Jain; Yuri P Vedernikov; Radek Bukowski; Robert E Garfield; Gary D Hankins; Garland D Anderson; George R Saade Journal: Am J Physiol Regul Integr Comp Physiol Date: 2004-12-30 Impact factor: 3.619
Authors: Maged M Costantine; Francesca Ferrari; Giusseppe Chiossi; Esther Tamayo; Gary D V Hankins; George R Saade; Monica Longo Journal: Am J Obstet Gynecol Date: 2009-07-26 Impact factor: 8.661