Literature DB >> 17401331

Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.

R Daniel Gietz1, Robert H Schiestl.   

Abstract

Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2-3 h, once the yeast cells have been grown depending on the heat shock used.

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Year:  2007        PMID： 17401331     DOI： 10.1038/nprot.2007.16

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  21 in total

1.  Compensation for differences in gene copy number among yeast ribosomal proteins is encoded within their promoters.

Authors:  Danny Zeevi; Eilon Sharon; Maya Lotan-Pompan; Yaniv Lubling; Zohar Shipony; Tali Raveh-Sadka; Leeat Keren; Michal Levo; Adina Weinberger; Eran Segal
Journal:  Genome Res       Date:  2011-10-18       Impact factor: 9.043

2.  Manipulating nucleosome disfavoring sequences allows fine-tune regulation of gene expression in yeast.

Authors:  Tali Raveh-Sadka; Michal Levo; Uri Shabi; Boaz Shany; Leeat Keren; Maya Lotan-Pompan; Danny Zeevi; Eilon Sharon; Adina Weinberger; Eran Segal
Journal:  Nat Genet       Date:  2012-05-27       Impact factor: 38.330

3.  Deciphering the rules by which 5'-UTR sequences affect protein expression in yeast.

Authors:  Shlomi Dvir; Lars Velten; Eilon Sharon; Danny Zeevi; Lucas B Carey; Adina Weinberger; Eran Segal
Journal:  Proc Natl Acad Sci U S A       Date:  2013-07-05       Impact factor: 11.205

4.  The toxicity of an "artificial" amyloid is related to how it interacts with membranes.

Authors:  Julien Couthouis; Christelle Marchal; Fabien D'Angelo; Karine Berthelot; Christophe Cullin
Journal:  Prion       Date:  2010-10-23       Impact factor: 3.931

5.  Overexpression of the CORVET complex alleviates the fungicidal effects of fludioxonil on the yeast Saccharomyces cerevisiae expressing hybrid histidine kinase 3.

Authors:  Anmoldeep Randhawa; Debasree Kundu; Anupam Sharma; Rajendra Prasad; Alok K Mondal
Journal:  J Biol Chem       Date:  2018-11-16       Impact factor: 5.157

6.  Engineering a Model Cell for Rational Tuning of GPCR Signaling.

Authors:  William M Shaw; Hitoshi Yamauchi; Jack Mead; Glen-Oliver F Gowers; David J Bell; David Öling; Niklas Larsson; Mark Wigglesworth; Graham Ladds; Tom Ellis
Journal:  Cell       Date:  2019-04-04       Impact factor: 41.582

7.  Multiple signals converge on a differentiation MAPK pathway.

Authors:  Colin A Chavel; Heather M Dionne; Barbara Birkaya; Jyoti Joshi; Paul J Cullen
Journal:  PLoS Genet       Date:  2010-03-19       Impact factor: 5.917

8.  The tRNA modification complex elongator regulates the Cdc42-dependent mitogen-activated protein kinase pathway that controls filamentous growth in yeast.

Authors:  Ummi Abdullah; Paul J Cullen
Journal:  Eukaryot Cell       Date:  2009-07-24

9.  A genome-wide screen in yeast identifies specific oxidative stress genes required for the maintenance of sub-cellular redox homeostasis.

Authors:  Anita Ayer; Sina Fellermeier; Christopher Fife; Simone S Li; Gertien Smits; Andreas J Meyer; Ian W Dawes; Gabriel G Perrone
Journal:  PLoS One       Date:  2012-09-06       Impact factor: 3.240

10.  A simple yeast-based strategy to identify host cellular processes targeted by bacterial effector proteins.

Authors:  Eran Bosis; Dor Salomon; Guido Sessa
Journal:  PLoS One       Date:  2011-11-15       Impact factor: 3.240
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