Literature DB >> 17401146

Expression in Pichia pastoris and immunological evaluation of a truncated Dengue envelope protein.

Iris Valdés1, Lisset Hermida, Aída Zulueta, Jorge Martín, Ricardo Silva, Mayling Alvarez, María G Guzmán, Gerardo Guillén.   

Abstract

Among the Dengue virus structural proteins, the Envelope glycoprotein is the most important because of its antigenic characteristics. In this work, the E protein from Dengue-2 virus truncated at the C-terminus region was successfully expressed in Pichia pastoris. The E2trunc gene was cloned under the AOX1 promoter from P. pastoris and the signal peptide of the sucrose invertase gene from Saccharomyces cerevisiae. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of expression revealed the presence of a protein with the expected size, which was completely associated to the insoluble fraction after cellular disruption. The recombinant N-glycosylated protein reacted with two conformational antibodies against Dengue-2, indicating a proper folding of it. In addition, it was able to induce antiviral antibodies after mice immunization.

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Year:  2007        PMID： 17401146     DOI： 10.1385/mb:35:1:23

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  15 in total

1.  A dengue-2 Envelope fragment inserted within the structure of the P64k meningococcal protein carrier enables a functional immune response against the virus in mice.

Authors:  Lisset Hermida; Rayner Rodríguez; Laura Lazo; Ricardo Silva; Aída Zulueta; Glay Chinea; Carlos López; María G Guzmán; Gerardo Guillén
Journal:  J Virol Methods       Date:  2004-01       Impact factor: 2.014

2.  The fusion site of envelope fragments from each serotype of Dengue virus in the P64k protein, influence some parameters of the resulting chimeric constructs.

Authors:  Aída Zulueta; Lisset Hermida; Laura Lazo; Iris Valdés; Rayner Rodríguez; Carlos López; Ricardo Silva; Delfina Rosario; Jorge Martín; María G Guzmán; Gerardo Guillén
Journal:  Biochem Biophys Res Commun       Date:  2003-08-29       Impact factor: 3.575

3.  Purification of native dengue-2 viral proteins and the ability of purified proteins to protect mice.

Authors:  R Feighny; J Burrous; J McCown; C Hoke; R Putnak
Journal:  Am J Trop Med Hyg       Date:  1992-10       Impact factor: 2.345

4.  Recombinant dengue virus type 2 envelope/hepatitis B surface antigen hybrid protein expressed in Pichia pastoris can function as a bivalent immunogen.

Authors:  Himani Bisht; Dipti A Chugh; Manoj Raje; S Swaminathan Swaminathan; Navin Khanna
Journal:  J Biotechnol       Date:  2002-10-23       Impact factor: 3.307

5.  Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaica.

Authors:  J T Roehrig; R A Bolin; R G Kelly
Journal:  Virology       Date:  1998-07-05       Impact factor: 3.616

6.  Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles.

Authors:  R J Sugrue; J Fu; J Howe; Y C Chan
Journal:  J Gen Virol       Date:  1997-08       Impact factor: 3.891

7.  Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full-length genome.

Authors:  V Deubel; R M Kinney; D W Trent
Journal:  Virology       Date:  1988-07       Impact factor: 3.616

8.  A ligand-binding pocket in the dengue virus envelope glycoprotein.

Authors:  Yorgo Modis; Steven Ogata; David Clements; Stephen C Harrison
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-20       Impact factor: 11.205

Review 9.  Recent advances in the expression of foreign genes in Pichia pastoris.

Authors:  J M Cregg; T S Vedvick; W C Raschke
Journal:  Biotechnology (N Y)       Date:  1993-08

10.  Dengue haemorrhagic fever--a public health problem and a field for research.

Authors:  S B Halstead
Journal:  Bull World Health Organ       Date:  1980       Impact factor: 9.408
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  5 in total

Review 1.  Recent Developments in Recombinant Protein-Based Dengue Vaccines.

Authors:  Nagesh K Tripathi; Ambuj Shrivastava
Journal:  Front Immunol       Date:  2018-08-23       Impact factor: 7.561

2.  Conformational and thermal stability improvements for the large-scale production of yeast-derived rabbit hemorrhagic disease virus-like particles as multipurpose vaccine.

Authors:  Erlinda Fernández; Jorge R Toledo; Lídice Méndez; Nemecio González; Francisco Parra; José M Martín-Alonso; Miladys Limonta; Kosara Sánchez; Ania Cabrales; Mario P Estrada; Alina Rodríguez-Mallón; Omar Farnós
Journal:  PLoS One       Date:  2013-02-27       Impact factor: 3.240

3.  Pichia pastoris-expressed dengue 2 envelope forms virus-like particles without pre-membrane protein and induces high titer neutralizing antibodies.

Authors:  Shailendra Mani; Lav Tripathi; Rajendra Raut; Poornima Tyagi; Upasana Arora; Tarani Barman; Ruchi Sood; Alka Galav; Wahala Wahala; Aravinda de Silva; Sathyamangalam Swaminathan; Navin Khanna
Journal:  PLoS One       Date:  2013-05-23       Impact factor: 3.240

4.  A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization.

Authors:  Rochelle Aw; Paul F McKay; Robin J Shattock; Karen M Polizzi
Journal:  Protein Expr Purif       Date:  2018-03-27       Impact factor: 1.650

5.  Avian influenza A/H5N1 neuraminidase expressed in yeast with a functional head domain.

Authors:  S Yongkiettrakul; K Boonyapakron; A Jongkaewwattana; A Wanitchang; U Leartsakulpanich; P Chitnumsub; L Eurwilaichitr; Y Yuthavong
Journal:  J Virol Methods       Date:  2008-12-11       Impact factor: 2.014
  5 in total

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