Literature DB >> 17400298

Genotype differentiating RT-PCR and sandwich ELISA: handy tools in epidemiological investigation of foot and mouth disease.

Jajati Keshari Mohapatra1, Saravanan Subramaniam, Chakradhar Tosh, Divakar Hemadri, Aniket Sanyal, Tamilselvan Ramasamy Periyasamy, Thaha Jamal Rasool.   

Abstract

Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.

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Year:  2007        PMID: 17400298     DOI: 10.1016/j.jviromet.2007.02.008

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  4 in total

Review 1.  Diagnostic assays developed for the control of foot-and-mouth disease in India.

Authors:  Gaurav Kumar Sharma; Sonalika Mahajan; Rakesh Matura; Saravanan Subramaniam; Rajeev Ranjan; Jitendra Biswal; Manoranjan Rout; Jajati Keshari Mohapatra; Bana Bihari Dash; Aniket Sanyal; Bramhadev Pattnaik
Journal:  World J Virol       Date:  2015-08-12

2.  Foot-and-mouth disease virus antigen detection enzyme-linked immunosorbent assay using multiserotype-reactive monoclonal antibodies.

Authors:  Kazuki Morioka; Katsuhiko Fukai; Kazuo Yoshida; Reiko Yamazoe; Hiroyuki Onozato; Seiichi Ohashi; Tomoyuki Tsuda; Kenichi Sakamoto
Journal:  J Clin Microbiol       Date:  2009-09-16       Impact factor: 5.948

3.  Development and Evaluation of a Rapid Antigen Detection and Serotyping Lateral Flow Antigen Detection System for Foot-and-Mouth Disease Virus.

Authors:  Kazuki Morioka; Katsuhiko Fukai; Kazuo Yoshida; Rie Kitano; Reiko Yamazoe; Manabu Yamada; Tatsuya Nishi; Toru Kanno
Journal:  PLoS One       Date:  2015-08-13       Impact factor: 3.240

4.  Evaluation of monoclonal antibody-based sandwich direct ELISA (MSD-ELISA) for antigen detection of foot-and-mouth disease virus using clinical samples.

Authors:  Kazuki Morioka; Katsuhiko Fukai; Kenichi Sakamoto; Kazuo Yoshida; Toru Kanno
Journal:  PLoS One       Date:  2014-04-15       Impact factor: 3.240

  4 in total

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