| Literature DB >> 17400003 |
Wenna Bao1, Haifeng Pan, Min Lu, Yuan Ni, Rui Zhang, Xingguo Gong.
Abstract
Sarsasapogenin, a kind of mainly effective components of Anemarrhena asphodeloides Bunge (Liliaceae) has the effects of being anti-diabetes and improving memory. However, there are few reports focusing on its anti-tumor effects. In this study, the sarsasapogenin was extracted from rhizomes of A. asphodeloides Bunge and applied to inhibit HepG2 human hepatoma cells. MTT assay showed that sarsasapogenin induced a distinct dose- and time-dependent diminution of cell viability with IC(50) of 42.4+/-1.0microg/ml for 48h. Furthermore, sarsasapogenin-induced apoptosis of HepG2 cells was verified by Hoechst 33258 staining, electron microscopy, DNA fragmentation and PI staining. Flow cytometry analysis showed that sarsasapogenin-induced cell apoptosis was through arrest of cell cycle in G(2)/M phase. Hence we proposed that sarsasapogenin could be used as an anti-liver cancer drug for future studies.Entities:
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Year: 2007 PMID： 17400003 DOI： 10.1016/j.cellbi.2007.02.001
Source DB: PubMed Journal: Cell Biol Int ISSN： 1065-6995 Impact factor: 3.612