BACKGROUND: Cellular cardiomyoplasty with bone marrow derived stromal (MSC) and mononuclear (BMNC) cells has been shown to improve performance of infarcted hearts. We performed a comparative study with MSC and BMNC and tested the hypothesis that captopril treatment could enhance the beneficial effect of cell therapy in large myocardial infarctions. METHODS: Male syngeneic Wistar rats underwent experimental infarction and were randomized to receive 1-3 x 10(6) MSC, 10(8) BMNC or vehicle (BSS group). Two additional groups were treated with captopril and received 1-3 x 10(6) MSC (Cap.MSC) or vehicle (Cap). RESULTS: The ejection fraction (EF%) of MSC and BMNC-treated rats was higher than in the BSS rats, eight weeks after transplantation (33.0+/-4.0, 34.0+/-2.0 and 20.0+/-2.0% respectively, P<0.01). Both captopril-treated groups improved EF% similarly. But only captopril plus MSC treatment almost restored cardiac function to control levels, 8 weeks after injection (60.50+/-5.40% vs. 41.00+/-4.50% in Cap.MSC and Cap respectively, P<0.05). Many DAPI-labelled cells were found in the scar tissue of the left ventricle only in the Cap.MSC group. CONCLUSIONS: Cell transplantation with both MSC and BMNC produced a similar stabilisation of heart function, but the success of the cell engraftment and the recovery of cardiac performance were dependent on concomitant treatment with captopril.
BACKGROUND:Cellular cardiomyoplasty with bone marrow derived stromal (MSC) and mononuclear (BMNC) cells has been shown to improve performance of infarcted hearts. We performed a comparative study with MSC and BMNC and tested the hypothesis that captopril treatment could enhance the beneficial effect of cell therapy in large myocardial infarctions. METHODS: Male syngeneic Wistar rats underwent experimental infarction and were randomized to receive 1-3 x 10(6) MSC, 10(8) BMNC or vehicle (BSS group). Two additional groups were treated with captopril and received 1-3 x 10(6) MSC (Cap.MSC) or vehicle (Cap). RESULTS: The ejection fraction (EF%) of MSC and BMNC-treated rats was higher than in the BSS rats, eight weeks after transplantation (33.0+/-4.0, 34.0+/-2.0 and 20.0+/-2.0% respectively, P<0.01). Both captopril-treated groups improved EF% similarly. But only captopril plus MSC treatment almost restored cardiac function to control levels, 8 weeks after injection (60.50+/-5.40% vs. 41.00+/-4.50% in Cap.MSC and Cap respectively, P<0.05). Many DAPI-labelled cells were found in the scar tissue of the left ventricle only in the Cap.MSC group. CONCLUSIONS: Cell transplantation with both MSC and BMNC produced a similar stabilisation of heart function, but the success of the cell engraftment and the recovery of cardiac performance were dependent on concomitant treatment with captopril.
Authors: César R M Costa; Fernando A C Seara; Milena S Peixoto; Isalira P Ramos; Raiana A Q Barbosa; Adriana B Carvalho; Rodrigo S Fortunato; Anderson L B Silveira; Emerson L Olivares Journal: Mol Biol Rep Date: 2020-10-13 Impact factor: 2.316
Authors: Bruno M Andrade; Marcelo R Baldanza; Karla C Ribeiro; Anderson Porto; Ramon Peçanha; Fabio S A Fortes; Gisele Zapata-Sudo; Antonio C Campos-de-Carvalho; Regina C S Goldenberg; João Pedro Werneck-de-Castro Journal: PLoS One Date: 2015-06-03 Impact factor: 3.240
Authors: F A C Seara; I G Araujo; G E Império; M P Marassi; A C M Silva; A S Mecawi; L C Reis; E L Olivares Journal: Braz J Med Biol Res Date: 2019-10-10 Impact factor: 2.590
Authors: Carolyn A Carr; Daniel J Stuckey; Louise Tatton; Damian J Tyler; Sarah J M Hale; Dominic Sweeney; Jürgen E Schneider; Enca Martin-Rendon; George K Radda; Sian E Harding; Suzanne M Watt; Kieran Clarke Journal: Am J Physiol Heart Circ Physiol Date: 2008-06-06 Impact factor: 4.733