Literature DB >> 17397937

Implementation of a one-step real-time RT-PCR protocol for diagnosis of foot-and-mouth disease.

Andrew E Shaw1, Scott M Reid, Katja Ebert, Geoffrey H Hutchings, Nigel P Ferris, Donald P King.   

Abstract

An automated one-step real-time reverse transcription polymerase chain reaction (rRT-PCR) protocol was optimised and evaluated for the routine diagnosis of foot-and-mouth disease (FMD). Parallel testing of RNA samples (n=257) indicated that this assay has a diagnostic sensitivity at least equivalent to the automated two-step rRT-PCR protocol previously used for the laboratory detection of FMD virus (FMDV). This more rapid and economical one-step protocol will play a key role in contingency planning for any future outbreaks of FMD in the United Kingdom (UK).

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Year:  2007        PMID： 17397937     DOI： 10.1016/j.jviromet.2007.02.009

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  41 in total

1.  Simultaneous detection of three arboviruses using a triplex RT-PCR: enzyme hybridization assay.

Authors:  Dan Dong; Shi-hong Fu; Li-hua Wang; Zhi Lv; Tai-yuan Li; Guo-dong Liang
Journal:  Virol Sin       Date:  2012-06-09       Impact factor: 4.327

2.  Immune Response in Goats to Different Payloads of FMDV Monovalent Vaccine: Protection Against Virulent Challenge and Development of Carrier Status.

Authors:  M Madhanmohan; S B Nagendrakumar; P Santhakumar; D Thiagarajan; M Lakshmi Narasu; V A Srinivasan
Journal:  Indian J Microbiol       Date:  2011-01-25       Impact factor: 2.461

3.  Protection against direct in-contact challenge following foot-and-mouth disease vaccination in sheep and goats: the effect on virus excretion and carrier status.

Authors:  Muthukrishnan Madhanmohan; Singanallur Balasubramanian Nagendrakumar; Villuppanoor Alwar Srinivasan
Journal:  Vet Res Commun       Date:  2010-03-30       Impact factor: 2.459

4.  Ribavirin-resistant variants of foot-and-mouth disease virus: the effect of restricted quasispecies diversity on viral virulence.

Authors:  Jianxiong Zeng; Haiwei Wang; Xiaochun Xie; Chen Li; Guohui Zhou; Decheng Yang; Li Yu
Journal:  J Virol       Date:  2014-01-22       Impact factor: 5.103

5.  A Temperature-Dependent Translation Defect Caused by Internal Ribosome Entry Site Mutation Attenuates Foot-and-Mouth Disease Virus: Implications for Rational Vaccine Design.

Authors:  Decheng Yang; Chao Sun; Rongyuan Gao; Haiwei Wang; Wenming Liu; Kewei Yu; Guohui Zhou; Bo Zhao; Li Yu
Journal:  J Virol       Date:  2020-07-30       Impact factor: 5.103

6.  Loop-mediated isothermal amplification (RT-LAMP): a new approach for the detection of foot-and-mouth disease virus and its sero-types in Pakistan.

Authors:  U Farooq; A Latif; H Irshad; A Ullah; A B Zahur; K Naeem; S U H Khan; Z Ahmed; L L Rodriguez; G Smoliga
Journal:  Iran J Vet Res       Date:  2015       Impact factor: 1.376

7.  Polymorphisms of the IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein.

Authors:  Renjin Chen; Zhangping Yang; Dejun Ji; Yongjiang Mao; Ying Chen; Yunlong Li; Haitao Wu; Xiaolong Wang; Lingling Chang
Journal:  Mol Biol Rep       Date:  2010-11-27       Impact factor: 2.316

8.  The development of a rapid SYBR one step real-time RT-PCR for detection of Porcine Reproductive and Respiratory Syndrome Virus.

Authors:  Hong Tian; JingYan Wu; YouJun Shang; Yan Cheng; XiangTao Liu
Journal:  Virol J       Date:  2010-05-10       Impact factor: 4.099

9.  Diagnostic evaluation of multiplexed reverse transcription-PCR microsphere array assay for detection of foot-and-mouth and look-alike disease viruses.

Authors:  Benjamin J Hindson; Scott M Reid; Brian R Baker; Katja Ebert; Nigel P Ferris; Lance F Bentley Tammero; Raymond J Lenhoff; Pejman Naraghi-Arani; Elizabeth A Vitalis; Thomas R Slezak; Pamela J Hullinger; Donald P King
Journal:  J Clin Microbiol       Date:  2008-01-23       Impact factor: 5.948

10.  Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.

Authors:  John G Bruno; Maria P Carrillo; Taylor Phillips
Journal:  J Biomol Tech       Date:  2008-04
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