Literature DB >> 17393288

Hydroxypropyl-beta-cyclodextrin enhanced determination for the Vitamin B12 by fluorescence quenching method.

Jing Sun1, Xiashi Zhu, Ming Wu.   

Abstract

A novel fluorescence quenching method for the determination of Vitamin B12(VB12) had been developed. It was based on that the fluorescence intensity of erythrosine sodium(ES) could be enhanced by Hydroxypropyl-beta-cyclodextrin(HP-beta-CD) due to the formation of inclusion complex (HP-beta-CD-ES), while the fluorescence intensity of HP-beta-CD-ES was diminished after adding VB12 into the system, and there was a linear relationship between the fluorescence quenching value of the system (DeltaF) and the concentration of VB12 (c). The mechanism of the determination of VB12 was discussed. The results showed that under the optimal conditions, the linear range of calibration curve for the determination of VB12 was 0.0 approximately 2.1 x 10(-5) mol/L, and the detection limit was 1.8 x 10(-7) mol/ L. It could be satisfactorily applied to the determination of VB12 in injections.

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Year:  2007        PMID： 17393288     DOI： 10.1007/s10895-007-0168-2

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.525


  6 in total

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  6 in total
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