Literature DB >> 17393098

Egr-1 mediates Si0(2)-driven transcription of membrane type I matrix metalloproteinase in macrophages.

Fei Xiang1, Ming Bai, Yang Jin, Wanli Ma, Jianbao Xin.   

Abstract

The up-regulation mechanism of membrane type I matrix metalloproteinase (MT1-MMP) in macrophages stimulated by silica in vitro and the contribution of early growth response 1 (Egr-1) transcription factor in the gene expression pathway were investigated. Macrophages stimulated by silica were treated with Egr-1 antibody or Egr-1 decoy oligodeoxynucleotides (ODN). The levels of MT1-MMP proteins were determined by Western blot and the expression of MT1-MMP mRNAs was detected by RT-PCR. The results showed as compared with control macrophages, silica-stimulated group showed up-regulated gene expression of MT1-MMP via Egr-1 (P<0.01). Compared with silica-stimulated macrophages untreated with antibody, the cells treated with 5 microg/mL Egr-1 antibody were associated with reduced expression of MT1-MMP protein (P<0.01) and mRNA (P<0.01). Compared with silica-stimulated untransfected group, the Egr-1 "decoy" ODN group was associated with reduction in the expression of MT1-MMP protein and mRNA (P<0.01). It was concluded gene expression of MT1-MMP which may play a critical role in silicosis was up-regulated by silica in macrophages. Egr-1 participated in the expression of MT1-MMP and positively regulated the expression. Both Egr-1 antibody and Egr-1 decoy ODN suppressed the expression of MT1-MMP through the Egr-1 pathway and may become a potential therapeutic tool in the management of silicosis in the future.

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Year:  2007        PMID： 17393098     DOI： 10.1007/s11596-007-0104-3

Source DB:  PubMed          Journal:  J Huazhong Univ Sci Technolog Med Sci        ISSN： 1672-0733


  15 in total

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Authors:  J L Kang; Y H Go; K C Hur; V Castranova
Journal:  J Toxicol Environ Health A       Date:  2000-05-12

2.  Regulation of osteoclastogenesis by antisense oligodeoxynucleotides specific to zinc finger nuclear transcription factors Egr-1 and WT1 in rat bone marrow culture system.

Authors:  T Kukita; A Kukita; H Harada; T Iijima
Journal:  Endocrinology       Date:  1997-10       Impact factor: 4.736

3.  Bleomycin upregulates gene expression of angiotensin-converting enzyme via mitogen-activated protein kinase and early growth response 1 transcription factor.

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Journal:  Am J Respir Cell Mol Biol       Date:  2001-11       Impact factor: 6.914

4.  Macrophage-derived MT1-MMP and increased MMP-2 activity are associated with glomerular damage in crescentic glomerulonephritis.

Authors:  K Hayashi; S Horikoshi; S Osada; K Shofuda; I Shirato; Y Tomino
Journal:  J Pathol       Date:  2000-07       Impact factor: 7.996

5.  Egr-1 mediates extracellular matrix-driven transcription of membrane type 1 matrix metalloproteinase in endothelium.

Authors:  T L Haas; D Stitelman; S J Davis; S S Apte; J A Madri
Journal:  J Biol Chem       Date:  1999-08-06       Impact factor: 5.157

6.  Cellular mechanisms for human procollagenase-3 (MMP-13) activation. Evidence that MT1-MMP (MMP-14) and gelatinase a (MMP-2) are able to generate active enzyme.

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Journal:  J Biol Chem       Date:  1996-07-19       Impact factor: 5.157

7.  Matrix metalloproteinase-2: mechanism and regulation of NF-kappaB-mediated activation and its role in cell motility and ECM-invasion.

Authors:  Subha Philip; Anuradha Bulbule; Gopal C Kundu
Journal:  Glycoconj J       Date:  2004       Impact factor: 2.916

Review 8.  Processing, shedding, and endocytosis of membrane type 1-matrix metalloproteinase (MT1-MMP).

Authors:  Pamela Osenkowski; Marta Toth; Rafael Fridman
Journal:  J Cell Physiol       Date:  2004-07       Impact factor: 6.384

9.  [Expression of early growth response gene-1 in macrophages stimulated by silicon dioxide].

Authors:  Ling Chu; Jin-wu Peng; Hai-ying Jiang; Qing-fu Zeng
Journal:  Zhonghua Bing Li Xue Za Zhi       Date:  2003-12

10.  A matrix metalloproteinase expressed on the surface of invasive tumour cells.

Authors:  H Sato; T Takino; Y Okada; J Cao; A Shinagawa; E Yamamoto; M Seiki
Journal:  Nature       Date:  1994-07-07       Impact factor: 49.962
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  3 in total

1.  Indications for distinct pathogenic mechanisms of asbestos and silica through gene expression profiling of the response of lung epithelial cells.

Authors:  Timothy N Perkins; Paul M Peeters; Arti Shukla; Ingrid Arijs; Julie Dragon; Emiel F M Wouters; Niki L Reynaert; Brooke T Mossman
Journal:  Hum Mol Genet       Date:  2014-10-27       Impact factor: 6.150

2.  A Transcriptional Regulatory Role for the Membrane Type-1 Matrix Metalloproteinase in Carcinogen-Induced Inflammasome Gene Expression.

Authors:  Samuel Sheehy; Borhane Annabi
Journal:  Gene Regul Syst Bio       Date:  2017-06-08

Review 3.  Extracellular signal-regulated kinase signaling pathway and silicosis.

Authors:  Yujia Xie; Jixuan Ma; Meng Yang; Lieyang Fan; Weihong Chen
Journal:  Toxicol Res (Camb)       Date:  2021-05-07       Impact factor: 3.524
  3 in total

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