W Shane Journeay1, K S Janardhan, B Singh. 1. Immunology Research Group, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada, S7N 5B4.
Abstract
OBJECTIVE AND DESIGN: We tested the hypothesis that total endothelial monocyte-activating polypeptide-II (EMAP-II) expression (proEMAP/p43 and mature EMAP-II) is up-regulated in lipopolysaccharide (LPS)-induced acute lung inflammation (ALI) and that mature EMAP-II induces monocyte/macrophage and granulocyte recruitment in vivo. MATERIALS: Thirty-five 10 week old, male Sprague-Dawley rats. TREATMENT: Animals were instilled intratracheally with 250 microg of E. coli LPS (N = 15) or saline (N = 5) or 20 microg of mature EMAP-II (N = 5). METHODS: Total EMAP-II was quantified using ELISA and the protein was localized with light and electron microscopic immunocytochemistry in lungs of rats at 1, 3 and 12 h (n = 5/group). RESULTS: ELISA showed increased total EMAP-II concentrations (p < 0.05) in lungs from LPS-treated rats compared to control animals. Compared to the controls, light and electron microscopic imunocytochemistry localized total EMAP-II in monocytes/macrophages and alveolar septa at 1 and 3 h and in vascular smooth muscles at 12 h post-LPS treatment. Instillation of mature EMAP-II increased lung monocytes/macrophages and granulocytes compared with control animals (p < 0.05). However, compared to the LPS treatment, mature EMAP-II instillation did not induce expression of IL-1beta and MIP-2 (p < 0.05) and provoked less vigorous recruitment of monocytes/macrophages. CONCLUSION: EMAP-II expression is increased in LPS-induced ALI, and that intra-tracheal instillation of mature EMAP-II induces recruitment of monocytes/macrophages and granulocytes into the lungs without stimulating IL-1beta or MIP-2 expression.
OBJECTIVE AND DESIGN: We tested the hypothesis that total endothelial monocyte-activating polypeptide-II (EMAP-II) expression (proEMAP/p43 and mature EMAP-II) is up-regulated in lipopolysaccharide (LPS)-induced acute lung inflammation (ALI) and that mature EMAP-II induces monocyte/macrophage and granulocyte recruitment in vivo. MATERIALS: Thirty-five 10 week old, male Sprague-Dawley rats. TREATMENT: Animals were instilled intratracheally with 250 microg of E. coliLPS (N = 15) or saline (N = 5) or 20 microg of mature EMAP-II (N = 5). METHODS: Total EMAP-II was quantified using ELISA and the protein was localized with light and electron microscopic immunocytochemistry in lungs of rats at 1, 3 and 12 h (n = 5/group). RESULTS: ELISA showed increased total EMAP-II concentrations (p < 0.05) in lungs from LPS-treated rats compared to control animals. Compared to the controls, light and electron microscopic imunocytochemistry localized total EMAP-II in monocytes/macrophages and alveolar septa at 1 and 3 h and in vascular smooth muscles at 12 h post-LPS treatment. Instillation of mature EMAP-II increased lung monocytes/macrophages and granulocytes compared with control animals (p < 0.05). However, compared to the LPS treatment, mature EMAP-II instillation did not induce expression of IL-1beta and MIP-2 (p < 0.05) and provoked less vigorous recruitment of monocytes/macrophages. CONCLUSION: EMAP-II expression is increased in LPS-induced ALI, and that intra-tracheal instillation of mature EMAP-II induces recruitment of monocytes/macrophages and granulocytes into the lungs without stimulating IL-1beta or MIP-2 expression.
Authors: Matthias Clauss; Robert Voswinckel; Gangaraju Rajashekhar; Ninotchka L Sigua; Heinz Fehrenbach; Natalia I Rush; Kelly S Schweitzer; Ali Ö Yildirim; Krzysztof Kamocki; Amanda J Fisher; Yuan Gu; Bilal Safadi; Sandeep Nikam; Walter C Hubbard; Rubin M Tuder; Homer L Twigg; Robert G Presson; Sanjay Sethi; Irina Petrache Journal: J Clin Invest Date: 2011-05-16 Impact factor: 14.808