Literature DB >> 17393

The highly electrophilic character of 4-chloro-7-nitrobenzofurazan and possible consequences for its application as a protein-labelling reagent.

B S Baines, G Allen, K Brocklehurst.   

Abstract

4-Chloro-7-nitrobenzofurazan possesses at least one highly electrophilic centre (C-6) that is much more reactive towards nucleophiles than position C-4, the irreversible alkylating site of this reagent. Possible consequences of the electrophilic character of 4-chloro-7-nitrobenzofurazan for its application as a protein-labelling reagent are discussed.

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Year:  1977        PMID： 17393      PMCID： PMC1164679          DOI： 10.1042/bj1630189

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  The reactivity of SH groups with a fluorogenic reagent.

Authors:  D J. Birkett; N C. Price; G K. Radda; A G. Salmon
Journal:  FEBS Lett       Date:  1970-02-25       Impact factor: 4.124

2.  Fluorescent and spin label probes of the environments of the sulfhydryl groups of porcine muscle adenylate kinase.

Authors:  N C Price; M Cohn; R H Schirmer
Journal:  J Biol Chem       Date:  1975-01-25       Impact factor: 5.157

3.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

4.  4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.

Authors:  M Shipton; T Stuchbury; K Brocklehurst
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

5.  Investigation of the active site of papain with fluorescent probes.

Authors:  G Allen; G Lowe
Journal:  Biochem J       Date:  1973-08       Impact factor: 3.857

6.  A method for determining the adenosine triphosphatase content of energy-transducing membranes. reaction of 4-chloro-7-nitrobenzofurazan with the adenosine triphosphatase of bovine heart submitochondrial particles.

Authors:  S J Ferguson; W J Lloyd; G K Radda
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

7.  Evaluation of benzofuroxan as a chromophoric oxidizing agent for thiol groups by using its reactions with papain, ficin, bromelain and low-molecular-weight thiols.

Authors:  M Shipton; T Stuchbury; K Brocklehurst
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857
  7 in total
  5 in total

1.  Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols.

Authors:  M Shipton; K Brocklehurst
Journal:  Biochem J       Date:  1977-12-01       Impact factor: 3.857

2.  Anomalous reaction of 4-chloro-7-nitrobenzofurazan with thiol compounds.

Authors:  K Nitta; S C Bratcher; M J Kronman
Journal:  Biochem J       Date:  1979-02-01       Impact factor: 3.857

3.  Ionization characteristics and chemical influences of aspartic acid residue 158 of papain and caricain determined by structure-related kinetic and computational techniques: multiple electrostatic modulators of active-centre chemistry.

Authors:  M A Noble; S Gul; C S Verma; K Brocklehurst
Journal:  Biochem J       Date:  2000-11-01       Impact factor: 3.857

4.  Differences between the electric fields of the catalytic sites of papain and actinidin detected by using the thiol-located nitrobenzofurazan label as a spectroscopic reporter group.

Authors:  K Brocklehurst; E Salih; T S Lodwig
Journal:  Biochem J       Date:  1984-06-01       Impact factor: 3.857

5.  Impairment of the catalytic activity of Escherichia coli ribonucleic acid polymerase by a unique reaction of 4-chloro-7-nitrobenzofurazan.

Authors:  S C Bratcher; K Nitta; M J Kronman
Journal:  Biochem J       Date:  1979-11-01       Impact factor: 3.857
  5 in total

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